Microscale Thermophoresis (MST)
Microscale thermophoresis (MST) is a biophysical technique that measures the strength of the interaction between two molecules by detecting variations in fluorescence signal as a result of an IR laser-induced temperature change.
Microscale thermophoresis (MST) (Dhont et al. 2007; Duhr and Braun 2006), as other methods used to characterize interaction parameters, utilizes differential detection of bound and unbound states of a given molecular specimen. Quantification of the absolute or relative amounts of bound and unbound state can be used to determine characteristic physical parameters that describe the chemical equilibrium of an interaction. Differentiation between molecular states (e.g., bound and unbound) of a molecule can be achieved by using techniques that measure size (gel electrophoresis, fluorescence correlation spectroscopy, fluorescence polarization), charge (electrophoretic mobility shift assay), or conformation...
- Rainard JM, Pandarakalam GC, McElroy SP (2018) Using microscale thermophoresis to characterize hits from high-throughput screening: a European lead factory perspective. SLAS Discov Adv Life Sci RD 23:225–241Google Scholar