Encyclopedia of Biophysics

Living Edition
| Editors: Gordon Roberts, Anthony Watts, European Biophysical Societies

Microscale Thermophoresis (MST)

  • Amit J. GuptaEmail author
  • Stefan Duhr
  • Philipp Baaske
Living reference work entry
DOI: https://doi.org/10.1007/978-3-642-35943-9_10063-1



Microscale thermophoresis (MST) is a biophysical technique that measures the strength of the interaction between two molecules by detecting variations in fluorescence signal as a result of an IR laser-induced temperature change.


Microscale thermophoresis (MST) (Dhont et al. 2007; Duhr and Braun 2006), as other methods used to characterize interaction parameters, utilizes differential detection of bound and unbound states of a given molecular specimen. Quantification of the absolute or relative amounts of bound and unbound state can be used to determine characteristic physical parameters that describe the chemical equilibrium of an interaction. Differentiation between molecular states (e.g., bound and unbound) of a molecule can be achieved by using techniques that measure size (gel electrophoresis, fluorescence correlation spectroscopy, fluorescence polarization), charge (electrophoretic mobility shift assay), or conformation...

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Copyright information

© European Biophysical Societies’ Association (EBSA) 2018

Authors and Affiliations

  1. 1.NanoTemper Technologies GmbHMunichGermany

Section editors and affiliations

  • Alan Cooper
    • 1
  1. 1.School of Chemistry, University of GlasgowGlasgowUK