Industrial Enzymes

  • David A. BasketterEmail author
  • Monika Raulf
Reference work entry


A wide range of enzymes is used in many industrial environments.

The primary risk that they present to human health is sensitization of the respiratory tract.

Proteolytic enzymes can give risk to skin irritation.

All enzymes have the potential to cause immunologic contact urticaria, although this is not common.


Enzymes Respiratory allergy Contact urticaria Risk management 


  1. Amaro C, Goossens A (2008) Immunological occupational contact urticaria and contact dermatitis from proteins: a review. Contact Dermatitis 58:67–75CrossRefGoogle Scholar
  2. Amin S, Lahti A, Maibach HI (2008a) Contact urticaria and contact urticaria syndrome (immediate contact reactions). In: Zhai H, Wilhelm K-P, Maibach HI (eds) Dermatotoxicology, 7th edn. CRC Press, Boca Raton, pp 525–536Google Scholar
  3. Amin S, Lauerma A, Maibach HI (2008b) Diagnostic tests in dermatology: patch and photopatch testing and contact urticaria. In: Zhai H, Wilhelm K-P, Maibach HI (eds) Dermatotoxicology, 7th edn. CRC Press, Boca Raton, pp 581–586Google Scholar
  4. Basketter DA, York M, McFadden JP et al (2004) Determination of skin irritation potential in the human 4-h patch test. Contact Dermatitis 51:1–4CrossRefGoogle Scholar
  5. Basketter DA, English J, Wakelin S et al (2008) Enzymes, detergents and skin: facts and fantasies. Br J Dermatol 158:1177–1181CrossRefGoogle Scholar
  6. Basketter DA, Broekhuizen C, Fieldsend M et al (2010) Defining occupational and consumer exposure limits for enzyme protein respiratory allergens under REACH. Toxicology 268:165–170CrossRefGoogle Scholar
  7. Basketter DA, Berg N, Kruszewski F, Sarlo KS (2012) The toxicology and immunology of detergent enzymes. J Immunotoxicol 9:314–319CrossRefGoogle Scholar
  8. Basketter DA, Kruszewski F, Mathieu S, Kirchner DB, Panepinto A, Fieldsend M, Siegert V, Barnes F, Bookstaff R, Simonsen M, Concoby B (2015) Managing the risk of occupation allergy and asthma due to enzymes in the detergent industry. J Occup Environ Hyg 12:432–437CrossRefGoogle Scholar
  9. Bernstein JA, Sarlo K (2006) Enzymes. In: Bernstein IL, Chan-Yeung M, Malo J-L, Bernstein DI (eds) Asthma in the workplace, 3rd edn. Taylor & Francis Group, New York, pp 377–392CrossRefGoogle Scholar
  10. Cullinan P, Harris JM, Newman Taylor AJ et al (2000) An outbreak of asthma in a modern detergent factory. Lancet 356:1899–1900CrossRefGoogle Scholar
  11. Draize JH, Woodard G, Calvery HO (1944) Methods for the study of imitation and toxicity of substances applied topically to the skin and mucous membranes. J Pharmacol Exp Ther 82:377–390Google Scholar
  12. Flindt ML (1969) Pulmonary disease due to inhalation of derivatives of Bacillus subtilis containing proteolytic enzyme. Lancet 1:1177–1181CrossRefGoogle Scholar
  13. HERA (2009a) Human and Environmental Risk Assessment 2005 α-amylases, cellulases and lipases, 1st edn. Accessed 9 June 2009
  14. HERA (2009b) Human and Environmental Risk Assessment 2007 subtilisins, 2nd edn. Accessed 9 June 2009
  15. Hjorth N, Roed-Petersen J (1976) Occupational protein contact dermatitis in food handlers. Contact Dermatitis 2:28–42CrossRefGoogle Scholar
  16. Jirova D, Basketter D, Liebsch M et al (2010) Comparison of human skin irritation patch test data with in vitro skin irritation assays and animal data. Contact Dermatitis 62:109–116CrossRefGoogle Scholar
  17. Kanerva L, Tarvainen K (1990) Allergic contact dermatitis and contact urticaria from cellulolytic enzymes. Am J Contact Dermatitis 1:244–245CrossRefGoogle Scholar
  18. Kanerva L, Vanhanen M (1999) Occupational protein contact dermatitis from glucoamylase. Contact Dermatitis 41:171–173CrossRefGoogle Scholar
  19. Kanerva L, Vanhanen M (2001) Occupational allergic contact urticaria and rhinoconjunctivitis from a detergent protease. Contact Dermatitis 45:49–51CrossRefGoogle Scholar
  20. Kanerva L, Vanhanen M, Tupasela O (1997) Occupational allergic contact urticaria from fungal but not bacterial alpha-amylase. Contact Dermatitis 36:306–307CrossRefGoogle Scholar
  21. Kanerva L, Vanhanen M, Tupasela O (1998) Occupational contact urticaria from cellulase enzyme. Contact Dermatitis 38:176–177CrossRefGoogle Scholar
  22. Laukkanen A, Ruoppi P, Semes S (2007) Lactase-induced occupational protein contact dermatitis and allergic rhinoconjunctivitis. Contact Dermatitis 57:89–93CrossRefGoogle Scholar
  23. Levin C, Warshaw E (2008) Protein contact dermatitis: allergens, pathogenesis and management. Dermatitis 19:241–251CrossRefGoogle Scholar
  24. Medeiros S, Alves R, Santo R et al (2006) Bakers eczema and occupational asthma. Contact Dermatitis 55:49Google Scholar
  25. Morren M, Janssens V, Doom-Goossens A et al (1993) α-amylase, a flour additive: an important cause of protein contact dermatitis in bakers. J Am Acad Dermatol 29:723–728CrossRefGoogle Scholar
  26. Nicholson PJ, Newman-Taylor AJ, Oliver P et al (2001) Current best practice for the health surveillance of enzyme workers in the soap and detergent industry. Occup Med (Lond) 51:81–92CrossRefGoogle Scholar
  27. OECD (1992) Organisation for economic cooperation and development. Guidelines for testing of chemicals no 406. Skin sensitization. Organisation for Economic Cooperation and Development, ParisCrossRefGoogle Scholar
  28. OECD (2002) Organisation for economic cooperation and development. Test guideline 429: the local lymph node assay. OECD, ParisGoogle Scholar
  29. Pepys J (1972) Laboratory methods in clinical allergy – skin tests for immediate, type I, allergic reactions. Proc R Soc Med 65:271–272PubMedPubMedCentralGoogle Scholar
  30. Pepys J, Longbottom JL, Hargreave FE et al (1969) Allergic reactions of the lungs to enzymes of Bacillus subtilis. Lancet 1:1181–1184CrossRefGoogle Scholar
  31. Peters G, Johnson GQ, Golembiewski A (2001) Safe use of detergent enzymes in the workplace. Appl Occup Environ Hyg 16:389–396CrossRefGoogle Scholar
  32. Robinson MK, Horn PA, Kawabata TT, Babcock LS, Fletcher ER, Sarlo K (1998) Use of the mouse intranasal test (MINT) to determine the allergenic potency of detergent enzymes: comparison to the guinea pig intratracheal (GPIT) test. Toxicol Sci 43:39–46PubMedGoogle Scholar
  33. Sarlo K (2003) Control of occupational asthma and allergy in the detergent industry. Ann Allergy Asthma Immunol 90:32–34CrossRefGoogle Scholar
  34. Schweigert MK, Mackenzie DP, Sarlo K (2000) Occupational asthma and allergy associated with the use of enzymes in the detergent industry – a review of the epidemiology, toxicology and methods of prevention. Clin Exp Allergy 30:1511–1518CrossRefGoogle Scholar
  35. Simesen B, Wide L, Zachariae H (1976) Prick and radioallergosorbent tests (RAST) to Alcalase in a population not exposed to enzyme detergents. Acta Allergol 31:71–77CrossRefGoogle Scholar
  36. Smith Pease CK, White IR, Basketter DA (2002) Skin as a route of exposure to protein allergens. Clin Exp Dermatol 27:296–300CrossRefGoogle Scholar
  37. Soto-Mera MT, Lopez-Rico MR, Filgueira JF et al (2000) Occupational allergy to papain. Allergy 55:983–984CrossRefGoogle Scholar
  38. Tarvainen K, Kanerva L, Tupasela O et al (1991) Allergy from cellulase and xylanase enzymes. Clin Exp Allergy 21:609–615CrossRefGoogle Scholar
  39. Wuthrich B (1996) Protein contact dermatitis. Br J Dermatol 135:332–333CrossRefGoogle Scholar
  40. Zachariae H, Thomsen K, Rasmussen OG (1973) Occupational enzyme dermatitis. Results of patch testing with Alcalase. Acta Derm Venereol 53:145–148PubMedGoogle Scholar

Copyright information

© Springer Nature Switzerland AG 2020

Authors and Affiliations

  1. 1.DABMEB Consultancy Ltd.SharnbrookUK
  2. 2.Institute for Prevention an Occupational Medicine of German Social Accident InsuranceInstitute of the Ruhr-Universität BochumBochumGermany

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