Ect2 (Epithelial Cell Transforming 2 Oncogene)
Synonyms
Historical Background
Ect2 is a guanine nucleotide exchange factor (GEF) of the Rho GTPases (Tatsumoto et al. 1999). It was isolated as a cDNA clone with capability of converting mouse fibroblasts to malignantly transformed cells (Miki et al. 1991, 1993). Transforming Ect2 variants lacked the N-terminal regulatory domain, and efficiently induced focus formation, anchorage-independent cell growth, and cell invasiveness in mouse fibroblasts. Injection of athymic nude mice with Ect2 transformants resulted in tumor formation. While full-length Ect2 is localized only in the nucleus, its oncogenic variants are detected in the cytoplasm as well. In M phase, Ect2 distributes in the entire cells after nuclear membrane breakdown, and is concentrated on mitotic spindles and then central spindles. In the last stage of M phase, Ect2 translocates to the midbody, which is formed between two emerging daughter cells. Perturbation of Ect2 function in M phase resulted in the inhibition of cytokinesis (cellular division) without major effects on mitosis (Tatsumoto et al. 1999). These findings established that Ect2 is a critical regulator of cytokinesis. Subsequent studies on Ect2 as well as the Rho negative regulator MgcRacGAP clarified the molecular mechanisms of cytokinesis (Yuce et al. 2005; Kamijo et al. 2006); chromosome separation initiates the formation of central spindles where MgcRacGAP and MKLP1 (mitosis-specific motor protein) form a tight complex. Recruitment of Ect2 to this complex activates Rho at the cell equator leading to cytokinesis.
Structural Properties
Schematic view of Ect2 protein: Full-length Ect2 molecule constitutes of the N-terminal regulatory and C-terminal catalytic domains as well as the central S domain. The major motifs are shown. Triangles denote nuclear localization signals. The region carried by oncogenic Ect2 is shown by a line
The N-terminal half of Ect2 contains the BRCA1 terminal repeat (BRCT) domains (Tatsumoto et al. 1999). Identification of the fission yeast cut5 gene revealed a new homology region between Cut5 and the N-terminal half of Ect2. This region consists of two BRCT domains. Cut5 contains four repeats, whereas Ect2 has two. Fission yeast cut5 mutants show a very peculiar phenotype: no coordination between mitosis and cytokinesis, which results in the induction of cytokinesis during mitosis. The BRCT domains of Ect2 have at least two functions. First, they associate with the catalytic domain of Ect2, which renders the molecule inactive. Both the BRCT domains are considered essential to this function, as the expression of both the repeats, but not the either single repeat blocks cytokinesis as a dominant-negative mutant of Ect2. The central S domain may function as a joint of the N- and C-terminal halves, and phosphorylation of the S domain is known to affect the configuration (Hara et al. 2006). Since the BRCT sequence is known to function as a phosphospecific protein-binding motif, the Ect2 BRCT domains may function after the C-terminal domain is phosphorylated. A second function of the Ect2 BRCT domains is to interact with the Rho regulator MgcRacGAP (Yuce et al. 2005). Presumably, the conversion of the closed Ect2 structure to the open form stimulates the binding to MgcRacGAP. Phosphorylation of MgcRacGAP by Plk1 also stimulates Ect2-MgcRacGAP association (Wolfe et al. 2009).
Biochemical Functions
Biochemical function of Ect2. Ect2 replaces bound GDP by GTP on Rho GTPases, and therefore activates Rho to specifically associate with Rho targets, which in turn transmits the signal further downstream to regulate actin remodeling
Biological Functions
Biological function of Ect2: At the metaphase–anaphase transition, Ect2 associates with MgcRacGAP, which forms a tight complex with MKLP1 at the central spindle. Subsequent activation of Rho, Citron kinase, and MRLC (myosin regulatory light chain) causes the contractile ring formation, leading to cytokinesis
Although Ect2 knockdown mainly inhibits cytokinesis, it also impairs the attachment of mitotic spindles to the kinetochores, leading to a delay of prometaphase and abnormal chromosomal separation (Oceguera-Yanez et al. 2005). MgcRacGAP is involved in this function as well. While RhoA is a critical molecular switch in cytokinesis regulation, Cdc42 mainly functions in chromosome alignment in this mitotic role of Ect2.
Association of Ect2 with the polarity complex activates Rac1, leading to malignant transformation. In normal cells, Ect2 is localized to the nucleus, whereas it associates with Par6 in the cytoplasm of some cancer cells
Regulation of Ect2 by Phosphorylation and Protein Associations
The central domain of Ect2, designated the S domain, functions as a joint of the catalytic C-terminal and the regulatory N-terminal halves. It contains several phosphorylation sites, which may function as switches for configuration changes (Miki et al. 2009). Phosphorylation at T341 of Ect2 by Cdk1 in M phase affects configuration of the molecule (open and closed forms) and may control the accessibility of Ect2-binding proteins (Hara et al. 2006). Phosphorylation of T412 by Cdk1 also induces binding of another M phase kinase Plk1, leading to phosphorylation of Ect2 by Plk1 (Niiya et al. 2006). It is known that this phosphorylation is required for the exchange activity of Ect2. Plk1 appears to have multiple functions in M phase. Its N-terminal half contains the regulatory Polo-box domain (PBD), which can associate with phosphorylated peptides. Cdk1 functions as a “priming kinase” in this case: Cdk1 phosphorylation stimulates Plk1 binding through the PBD domain, and the kinase domain of Plk1 subsequently phosphorylates the substrate that the Plk1 has bound. Through this mechanism, Plk1 is recruited to various cellular compartments during M phase. Plk1 phosphorylation of Ect2 and MgcRacGAP is critical for cytokinesis regulation. The N-terminal domain of Ect2 mainly consists of two BRCT repeats, which are capable of binding to phosphorylated proteins. This domain functions to localize Ect2 to the central spindle. On the other hand, Ect2 is in a closed conformation in its inactive state through the interaction of the N- and C-terminal halves. The phosphorylation status of T431 regulates the configuration. Inhibition of Plk1 by a chemical inhibitor prevents Ect2 association to the central spindle as well as binding to MgcRacGAP (Wolfe et al. 2009). Thus, Plk1 phosphorylates MgcRacGAP, which may help Ect2 to associate with MgcRacGAP. On the other hand, Ect2 is also phosphorylated by Plk1, which is required for its activity. Complex phosphorylation events might regulate cytokinesis through these protein kinases.
Involvement of Tumor Formation
Cytoplasmic mislocalization of Ect2 induces its transforming activity: (upper) Inactive Ect2 forms a closed structure and it is localized at the nucleus (red). (Lower) Oncogenic Ect2 (see Fig. 1) displays cytoplasmic localization although it also localizes to the nucleus. NLS, nuclear localization signal
Despite the cytoplasmic mislocalization of transforming variants of Ect2, deletion or rearrangement of the ECT2 gene in human cancers has not been reported. Recent analyses of huge number of human cancers using microarray analyses revealed that Ect2 is a prognostic marker of several cancers including breast cancer. It is also reported that Ect2 expression inversely correlates to prognosis of glioblastomas (Sano et al. 2006). As described above, Ect2 associates with the tertiary complex consisting of the polarity determinant protein Par6, an atypical protein kinase C, and a scaffold protein Par3 (Liu et al. 2004, 2006) (Fig. 4). The gene for an aPKC, PKCiota, is located at the proximity of the ECT2 gene in human chromosome 3. Both the loci are co-amplified in several cancers including non-small cell lung carcinomas (NSLCs). Interestingly, Ect2 is mislocalized to the cytoplasm in these cancer cell lines, reminiscent of the phenotype of transforming Ect2. When either Par6 or PKCiota is knocked down in these cells, however, Ect2 localization returns to the nucleus. Thus, upregulation of the polarity complex appears to retain Ect2 in the cytoplasm in these cancer cell lines. It is well established that the Rho family GTPases, Cdc42 and Rac1, are involved in cell polarity. Ect2 knockdown in these cells inhibits tumorigenicity and invasiveness, indicating that Ect2 activates the Rac1 pathway through the polarity complex in these cells leading to malignant transformation.
Space limitations preclude comprehensive referencing. See also references therein.
Summary
Ect2 was originally isolated as a cDNA clone with an ability to morphologically transform mouse fibroblasts. The catalytic domain (C-terminal half) catalyzes guanine nucleotide exchange on Rho GTPases, whereas the regulatory domain (N-terminal half) contains two BRCT domains, which regulate the catalytic activity and also function to interact with other signaling molecules such as MgcRacGAP. Ect2 is a critical regulator of cytokinesis. Ect2 also interacts with the polarity determinants containing Par3, Par6, aPKC, and Rac1. In some cancer cells, amplification of a chromosomal region containing the genes for Ect2 and aPKC results in overproduction of these proteins. Cytoplasmic localization of Ect2/Par complex appears to cause malignant transformation of the cells.
References
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