Encyclopedia of Signaling Molecules

2018 Edition
| Editors: Sangdun Choi

EXO1 (Exonuclease 1)

Reference work entry
DOI: https://doi.org/10.1007/978-3-319-67199-4_101686


Historical Background

Exonuclease 1 (EXO1) was first identified in Schizosaccharomyces pombe (Szankasi and Smith 1995) and belongs to the Rad2/XPG family, which is conserved in its nuclease domain through species (Szankasi and Smith 1995; Wilson et al. 1998). The nuclease domain is located at the NH2-terminus and contains two subdomains the N-domain (N) and the internal (I) domain separated by a spacer region (Fig. 1). The EXO1 gene product exerts a 5′ → 3′ exonuclease and 5′ flap endonuclease activity (Lee and Wilson 1999; Keijzers et al. 2015). In addition, the EXO1 protein exhibits 5′ → 3′ intrinsic RNase H activity (Qiu et al. 1999). EXO1 has high affinity for processing double stranded DNA breaks (DSB), nicks, gaps, and pseudo-Y structures and can resolve double Holliday junctions. EXO1 is expressed at low level, independently of the cell cycle progression or proliferative status of the human cell (El-Shemerly et al. 2005). However, in mice, Exo1 is highly expressed in testis, spleen, and at lower levels in thymus, lymph node, and bone marrow. In human, the EXO1 expression levels are high in the testis, thymus, colon, and placenta (recently reviewed in Keijzers et al. 2016). EXO1 interacts with cell cycle regulators PCNA and the 14-3-3 complex. Cell cycle regulator proliferating cell nuclear antigen (PCNA) interacts physically with the PIP-box domain (Fig. 1) and stimulates EXO1 in its exonuclease activity on double-stranded DNA in vitro. During the S-phase, PCNA co-localizes with EXO1 and mismatch repair (MMR) protein MutS homolog 2 to replication foci (Liberti et al. 2011).
EXO1 (Exonuclease 1), Fig. 1

An overview of human EXO1 protein indicating the interacting and functional domain/motif. PIN motif = par interaction motif; N and I are subdomains of nuclease domains; NLS nuclear localization signal of EXO1; PIP-box is the PCNA-interacting peptide box. MSH3, MSH2, and MLH1 are the protein-binding domains

EXO1 interacts directly with the seven 14-3-3 isoforms in vitro and is stimulated by two of the seven isoforms in its exonuclease activity (Andersen et al. 2012). Moreover, it was recently suggested that 14-3-3σ restrains EXO1 nuclease activity during resection by counteracting PCNA in its stimulation of EXO1 (Chen et al. 2015). EXO1 is not directly linked to disease, but some mutations in the gene may indirectly contribute to different cancers including colorectal cancer. Although EXO1 has a weak phenotype, the protein did gain interest by its versatile roles in DNA metabolic processes including MMR, DNA end resection, meiosis, globulin maturation, and telomere maintenance (recently reviewed in Keijzers et al. 2016) (Fig. 2).
EXO1 (Exonuclease 1), Fig. 2

Human EXO1 participates in various DNA metabolism pathways. In (a) and (f) the X or U/G represents a base mismatch or uracil. EXO1 removes the mismatch containing DNA strand from 5′ → 3′ from a pre-introduced nick/gap. In (c) the end resection pathway, EXO1 acts on a double stranded DNA ends to generate 3′ ssDNA overhang for following DNA recombination events. (d) EXO1 operates in the resolution of double Holliday junctions during meiosis to generate crossover products. EXO1 contributes to immunoglobulin maturation and (e) class switching recombination and (f) somatic hypermutation. In (g) the role of telomere maintenance in humans remains unclear

EXO1 in Mismatch Repair

EXO1 is the only known nuclease, which is active in MMR in human cells. The MMR is a post-replicative DNA repair system that repairs certain damaged bases, base mismatches and base insertions/deletions, and various sizes of DNA loops (IDL). EXO1 has conserved domains and interacts directly with MMR factor MutS homolog 3 (MSH3) at the NH2-terminus of EXO1 and with MutL homolog 1 (MLH1) and MSH2 at the COOH-terminus. During the replication process, in the S-phase of the cell cycle, the proteins EXO1, MSH2, and PCNA co-localize (Liberti et al. 2011). In humans, the MMR is carried out by the MutSα complex, a heterodimer of MSH2-MutS homolog 6 (MSH6), which recognizes mainly single-base mismatches or the MutSβ complex, a heterodimer of MSH2-MSH3, which recognizes IDL. The MutSα and β complexes operate by binding to the DNA lesion, followed by binding of the MutLα, a heterodimer of MLH1/postmeiotic segregation increased 2 (PMS2), which forms a ternary complex with the MutSα or Mutsβ at the base damage site. Next, PCNA and replication factor C (RFC) are recruited and stimulate the MutLα to nick the DNA by use of the intrinsic endonuclease activity in PMS2; these nicks are created both 3′ and 5′ to the lesion. Subsequently, EXO1 is recruited to the damaged site to excise the damaged base or during replication to remove the newly synthesized DNA containing the error in a MutSα or (β) and MutLα-dependent manner. Replication protein A (RPA) facilitates protection of the single-stranded DNA (ssDNA) intermediates during the repair process to prevent formation of secondary structures and DNA degradation. In addition, RPA is involved in regulating accessibility of EXO1 to the DNA, ensuring that excision is only allowed in the presence of a replication error. In a joint activity, PCNA and DNA polymerase δ and DNA polymerase ε resynthesize the DNA and DNA ligase I and finalize the process by ligation of the nick (Kunkel and Erie 2015).

EXO1 Recruitment and Resection of Double-Stranded DNA Ends

Recent reports indicate that EXO1 is recruited to DNA damage in a PAR-mediated (poly(ADP-ribosyl)ation (PARylation)) manner (Zhang et al. 2015; Cheruiyot et al. 2015). PAR is a posttranslational modification, synthetized by Poly(ADP-ribose) polymerase 1 (PARP1), which provide a PAR chain docking platform on EXO1 for additional DNA damage repair factors which are rapidly recruited to the DNA damage site (Tallis et al. 2014; Cheruiyot et al. 2015). A PAR interaction motif, also named PIN motif, is located at the NH2-terminus of EXO1 (Fig. 1) (Zhang et al. 2015; Cheruiyot et al. 2015). In vitro experiments demonstrated that PARP1 physically interacts with EXO1 and stimulates EXO1 in its 5′ excision activity an in vitro MMR assay (Liu et al. 2011). A PAR-binding motif (125ITHAMAHKVIK135) in EXO1 was predicted (Gagné et al. 2008), but mutation of two essential residues in the PAR-binding motif to alanine (125ITHAMAAAVIA135) failed to show a difference in recruitment of EXO1 to DNA damage (Cheruiyot et al. 2015). Zhang and colleagues showed that an EXO1 mutant, EXO1-R93G, abolished the interaction with PAR and recruitment in cells to dsDNA damage (Zhang et al. 2015). During this initial stage of damage association, EXO1 resection activity, 5′ exo, and 5′ flap activity are held inactive by PAR until its clearance by poly(ADP-ribose) glycohydrolase (PARG) and other repair factors including PCNA is recruited (Cheruiyot et al. 2015).

During the resection of DSBs, an interplay between the MRE11-RAD50-NBS1 (MRN) complex and EXO1 is well documented (Nimonkar et al. 2011). A recent study showed that EXO1 is essential for embryogenesis and the DNA damage response in mice Exo1 null/null with hypomorphic Nijmegen breaking syndrome 1 (Nbs1) allele (Rein et al. 2015). NBS1 is a partner in the MRN complex, and deletion of either of the genes in the MRN complex is lethal, while deletion of Exo1 has a minimal impact in cells or mice (Williams et al. 2002; Schaetzlein et al. 2013). Deletion of Exo1 in mice expressing a hypomorphic allele of Nbs1 (Nbs1ΔB/ΔB) leads to severe developmental impairment, embryonic death, and chromosomal instability (Rein et al. 2015). The combination of Nbs1ΔB/ΔB-/EXO1-depleted cells are strongly affected in DNA replication, DNA repair, checkpoint signaling, and DNA damage response (Rein et al. 2015). Human EXO1 interacts directly with the RecQ helicases RECQL1, BLM, and WRN and is stimulated in its nuclease activity (Fig. 3) (reviewed in Keijzers et al. 2014). Additionally, EXO1 is regulated at the DNA ends by proteins such as WRN, RPA, Ku70/80, or CtIP ((C-terminal-binding protein) (CtBP)-interacting protein) to avoid unlimited resection (Eid et al. 2010; Iannascoli et al. 2015). Cells depleted in WRN and treated with DNA topoisomerase 1 (TOP1) inhibitor, camptothecin, which creates single-strand breaks (SSB) and eventually DSBs, show an enhanced degradation and ssDNA formation at nascent strands by the combined action of MRE11 and EXO1, as opposed to the limited processing of nascent strands performed by DNA2 in wild-type cells (Iannascoli et al. 2015). This suggests that WRN exonuclease activity prevents unscheduled degradation by MRE11 and EXO1 and thereby supports the restart of stalled forks and limits chromosome breakage (Iannascoli et al. 2015).
EXO1 (Exonuclease 1), Fig. 3

Human EXO1 participates in various DNA metabolism pathways. During these processes EXO1 interacts physically with other proteins, which may or may not stimulate EXO1 nuclease activity

It has been suggested that DNA resection occurs via two routes. Either via a RPA-BLM-DNA2-MRN mediated route, where the MRN complex promotes recruitment of BLM to DNA end and RPA stimulates BLM in DNA helices unwinding to stimulate 5′ → 3′ resection by DNA2 (Nimonkar et al. 2011). The other 5′ → 3′ resection route is mediated by EXO1 and stimulated by BLM, MRN, and RPA in DNA resection (Nimonkar et al. 2011). The Ku70/80 heterodimer, the center molecule in the DNA repair pathway nonhomologous end-joining (NHEJ), possesses a high affinity for double-stranded DNA ends. The Ku70/80 heterodimer prevents over-resection by nucleases from the DNA ends by binding tightly to the DNA ends. Studies in yeast showed that Ku70/80 blocks EXO1-mediated DNA end resection at forked dsDNA substrates. Another protein which modulates EXO1 activity is CtIP; it interacts directly with EXO1 and inhibits its exonuclease activity in vitro (Eid et al. 2010). Microhomology-mediated end joining (MMEJ) is an error-prone double stranded break DNA repair mechanism, which targets long stretches of single-stranded DNA up to 25 nucleotides, and is thought to be more mutagenic than the NHEJ. The MMEJ can function independently of the NHEJ factors (Ku70/80, DNA-dependent protein kinase catalytic subunit (DNA-PKcs), Ligase IV, and X-ray cross complementation group 4 protein (XRCC4)) and is active during G2-, S-, or G1-phase in the cell cycle. The MMEJ double-stranded DNA repair pathway includes BLM, MRN, EXO1, DNA2, FEN1, DNA polymerase β, λ, or μ, Ligase I or Ligase III/XRCC1, and the MMR proteins and has additional roles in humans in meiosis and class switching (see below). The MMEJ repair pathway is less well characterized than other DNA repair pathways; however the role of BLM stimulated by the exonuclease EXO1 or DNA2 during the MMEJ is well documented.

EXO1 in Meiosis

EXO1 activity plays an important role during meiosis. Mice studies show that both Exo1Δ6/Δ6 (Exo1 deleted in exon 6) and Exo1 null/null male and female mice were infertile when mated with wild-type mice (Wei et al. 2003; Schaetzlein et al. 2013). The Exo1 null/null mice had smaller testes, but this reduction was not caused by a decreased body weight in adult males (Schaetzlein et al. 2013). The Exo1Δ6/Δ6 as well as Exo1 null/null male mice were severely depleted of spermatids and spermatozoa (Wei et al. 2003; Schaetzlein et al. 2013). Further analysis of spermatogenesis in Exo1 null/null mice revealed that only a very small number of spermatogenic cells progress through meiosis II, which was indicated by the very few spermatozoa that could be retrieved from the epididymis of Exo1 null/null (Schaetzlein et al. 2013), suggesting that mouse EXO1 requires its catalytic activity, either endo- or exo-activity, to progress through meiosis II. The presence of pachytene spermatocytes in both Exo1Δ6/Δ6 and Exo1 null/null male mice indicates that meiosis can progress through prophase I (Wei et al. 2003; Schaetzlein et al. 2013).

EXO1 in Immunoglobulin Maturation

Diversity in antibodies occurs in two phases in B cells. In pre-B cells, rearrangement of variable (V), diversity (D), and joining (J) gene segments (V(D)J) at the immunoglobulin heavy chain region is initiated by the recombination-activating genes 1 and 2 (RAG1 and RAG2). Immature B cells will be preceded by class switch recombination (CSR) in the S (switch) region. The process of CSR is initiated by activation-induced deaminase (AID). AID creates mutations by substituting cytosine for uracil in the Ig loci at well-defined regions encoding rearranged V genes on the heavy and light chain loci and S regions on the heavy chain locus. Next, the BER pathway enzyme uracil-DNA glycosylase (UNG), in addition to MMR enzymes, facilitates the removal of mismatched bases leading to DSBs and deletions in the S region (rearrangement), creating increased variability. The created DSBs during gene segment rearrangement are repaired by the NHEJ as well as by MMEJ. B cells deficient in NHEJ require MMR proteins MLH1, MSH2, and EXO1 during CSR (Eccleston et al. 2011). The Exo1Δ6/Δ6 mice predominantly developed lymphoma between 16 and 18 months compared to Exo1+/Δ6 and wild-type mice (Wei et al. 2003). Additional studies in the Exo1Δ6/Δ6 demonstrated that the EXO1-mutated mice have decreased CSR and alteration in SHM by normal AID functioning (Bardwell et al. 2004). Interestingly, the Exo1Δ6/Δ6 phenotype suggested that mutation in the V region connects EXO1 and MLH1 to CSR and somatic hypermutation (SHM) (Bardwell et al. 2004). These observations are supported by Sμ tandem repeat mice (SμTR−/− mice); when crossed with Mlh1 −/− or Exo1Δ6/Δ6, an increased switch junction microhomology was noted compared to Msh2 −/− mice (Eccleston et al. 2009). The Exo1 null/null showed that the EXO1 nuclease activity has minor roles in correction of replication errors during MMR and in the generation of mutations by somatic hypermutation at Ig heavy chain region (Schaetzlein et al. 2013). B cells of the Exo1 null/null male mice, stimulated by either LPS or LPS/IL-4, failed to induce efficient CSR to switch from IgM to IgG3 or to IgG1, but this was not caused by impaired cell proliferation (Schaetzlein et al. 2013). These observations suggest that the MLH1 and EXO1 are dispensable in the early steps of CSR or in the promotion of AID- and MMR-triggered DSBs at the switch regions but required in microhomology-mediated resection joining the DNA ends.

EXO1 in Telomere Maintenance

Maintenance of the 3′ end of telomeres is essential for genome stability. Telomeres are packaged in loop structures in telomere loops (T-loops) and protected by the shelterin complex, including telomeric repeat factor 1 and telomeric repeat factor 2 (TFR1 and TRF2), protection of telomeres protein 1 (POT1), repressor activator protein 1 (RAP1), (TRF1)-interacting nuclear factor 2 (TIN2), and telomere protection protein (TPP1), and cooperating with accessory proteins such as MRE11, Ku70/80, and helicases BLM, WRN, and RECQL4. The 3′ telomere end invades the paired DNA at the telomeric region and forms a protective loop, thus forming a three-stranded DNA displacement loop (also called D-loop).

Studies in mice showed that during the cell cycle, the 3′ overhang synthesis is differently regulated at the leading- and lagging-end telomere, by the nucleases Apollo and EXO1, which initiates formation of the 3′ overhang at the leading- but not at the lagging-end telomeres. POT1 regulates Apollo at the telomere end to avoid hyper-resection; however extensive resection of the telomere ends generates transient long 3′ overhangs in the S/G2 cell cycle phase (Wu et al. 2012). Interestingly, telomere-dysfunctional mice Exo1Δ6/Δ6/mTerc−/− deleted in EXO1 and telomerase RNA component (TERC) improve organ maintenance and life span (Schaetzlein et al. 2007).


EXO1 is a 5′ → 3′ exonuclease, a 5′ flap endonuclease, and a 5′ RNase activity (Lee and Wilson 1999; Qiu et al. 1999; Keijzers et al. 2015). EXO1 is generally expressed in most tissue. During replication EXO1 co-localizes with MMR repair protein MSH2 and cell cycle regulator PCNA (Liberti et al. 2011). The EXO1 protein participates in both MMR and DSB repair. Mice studies suggest roles of EXO1 in meiosis, specifically in the spermatogenesis development; however a contribution of EXO1 to human meiosis is unknown. In both humans and mice, EXO1 has important roles in the MMEJ repair pathway, which is essential in immunoglobulin development specifically in CSR. Studies in mice suggest that EXO1 contributes to the formation of the 3′ overhang at leading-end telomeres (Wu et al. 2012), but less is known on the roles of EXO1 in telomere maintenance in humans.


  1. Andersen SD, Keijzers G, Rampakakis E, Engels K, Luhn P, El-Shemerly M, Nielsen FC, Du Y, May A, Bohr VA, Ferrari S, Zannis-Hadjopoulos M, Fu H, Rasmussen LJ. 14-3-3 checkpoint regulatory proteins interact specifically with DNA repair protein human exonuclease 1 (hEXO1) via a semi-conserved motif. DNA Repair (Amst). 2012;11:267–77.CrossRefGoogle Scholar
  2. Bardwell PD, Woo CJ, Wei K, Li Z, Martin A, Sack SZ, Parris T, Edelmann W, Scharff MD. Altered somatic hypermutation and reduced class-switch recombination in exonuclease 1-mutant mice. Nat Immunol. 2004;5:224–9.PubMedCrossRefGoogle Scholar
  3. Chen X, et al. 14-3-3 proteins restrain the Exo1 nuclease to prevent overresection. J Biol Chem. 2015;290:12300–12.PubMedPubMedCentralCrossRefGoogle Scholar
  4. Cheruiyot A, Paudyal SC, Kim IK, Sparks M, Ellenberger T, Piwnica-Worms H, You Z. Poly(ADP-ribose)-binding promotes Exo1 damage recruitment and suppresses its nuclease activities. DNA Repair (Amst). 2015;35:106–15.CrossRefGoogle Scholar
  5. Eccleston J, Schrader CE, Yuan K, Stavnezer J, Selsing E. Class switch recombination efficiency and junction microhomology patterns in Msh2-, Mlh1-, and Exo1-deficient mice depend on the presence of mu switch region tandem repeats. J Immunol. 2009;183:1222–8.PubMedPubMedCentralCrossRefGoogle Scholar
  6. Eccleston J, Yan C, Yuan K, Alt FW, Selsing E. Mismatch repair proteins MSH2, MLH1, and EXO1 are important for class-switch recombination events occurring in B cells that lack nonhomologous end joining. J Immunol. 2011;186:2336–43.PubMedPubMedCentralCrossRefGoogle Scholar
  7. Eid W, Steger M, El-Shemerly M, Ferretti LP, Peña-Diaz J, König C, Valtorta E, Sartori AA, Ferrari S. DNA end resection by CtIP and exonuclease 1 prevents genomic instability. EMBO Rep. 2010;11:962–8.PubMedPubMedCentralCrossRefGoogle Scholar
  8. El-Shemerly M, Janscak P, Hess D, Jiricny J, Ferrari S. Degradation of human exonuclease 1b upon DNA synthesis inhibition. Cancer Res. 2005;65:3604–9.PubMedCrossRefGoogle Scholar
  9. Gagné JP, Isabelle M, Lo KS, Bourassa S, Hendzel MJ, Dawson VL, Dawson TM, Poirier GG. Proteome-wide identification of poly(ADP-ribose) binding proteins and poly(ADP-ribose)-associated protein complexes. Nucleic Acids Res. 2008;36:6959–76.PubMedPubMedCentralCrossRefGoogle Scholar
  10. Iannascoli C, Palermo V, Murfuni I, Franchitto A, Pichierri P. The WRN exonuclease domain protects nascent strands from pathological MRE11/EXO1-dependent degradation. Nucleic Acids Res. 2015;43:9788–803.PubMedPubMedCentralGoogle Scholar
  11. Keijzers G, Maynard S, Shamanna RA, Rasmussen LJ, Croteau DL, Bohr VA. The role of RecQ helicases in non-homologous end-joining. Crit Rev Biochem Mol Biol. 2014;49:463–72.PubMedPubMedCentralCrossRefGoogle Scholar
  12. Keijzers G, Bohr VA, Rasmussen LJ. Human exonuclease 1 (EXO1) activity characterization and its function on flap structures. Biosci Rep. 2015;35:e00206.PubMedPubMedCentralGoogle Scholar
  13. Keijzers G, Liu D, Rasmussen LJ. Exonuclease 1 and its versatile roles in DNA Repair. Crit Rev Biochem Mol Biol. 2016;51:440–51.Google Scholar
  14. Kunkel TA, Erie DA. Eukaryotic mismatch repair in relation to DNA replication. Annu Rev Genet. 2015;49:291–313.PubMedPubMedCentralCrossRefGoogle Scholar
  15. Lee BI, Wilson DM. The RAD2 domain of human exonuclease 1 exhibits 5″ to 3″ exonuclease and flap structure-specific endonuclease activities. J Biol Chem. 1999;274:37763–9.PubMedCrossRefGoogle Scholar
  16. Liberti SE, Andersen SD, Wang J, May A, Miron S, Perderiset M, Keijzers G, Nielsen FC, Charbonnier JB, Bohr VA, Rasmussen LJ. Bi-directional routing of DNA mismatch repair protein human exonuclease 1 to replication foci and DNA double strand breaks. DNA Repair (Amst). 2011;10:73–86.CrossRefGoogle Scholar
  17. Liu Y, Kadyrov FA, Modrich P. PARP-1 enhances the mismatch-dependence of 5'-directed excision in human mismatch repair in vitro. DNA Repair (Amst). 2011;10:1145–53.CrossRefGoogle Scholar
  18. Nimonkar AV, Genschel J, Kinoshita E, Polaczek P, Campbell JL, Wyman C, Modrich P, Kowalczykowski S. BLM-DNA2-RPA-MRN and EXO1-BLM-RPA-MRN constitute two DNA end resection machineries for human DNA break repair. Genes Dev. 2011;25:350–62.PubMedPubMedCentralCrossRefGoogle Scholar
  19. Qiu J, Qian Y, Frank P, Wintersberger U, Shen B. Saccharomyces cerevisiae RNase H(35) functions in RNA primer removal during lagging-strand DNA synthesis, most efficiently in cooperation with Rad27 nuclease. Mol Cell Biol. 1999;19:8361–71.PubMedPubMedCentralCrossRefGoogle Scholar
  20. Rein K, Yanez DA, Terré B, Palenzuela L, Aivio S, Wei K, Edelmann W, Stark JM, Stracker TH. EXO1 is critical for embryogenesis and the DNA damage response in mice with a hypomorphic Nbs1 allele. Nucleic Acids Res. 2015;43:7371–87.PubMedPubMedCentralCrossRefGoogle Scholar
  21. Schaetzlein S, Kodandaramireddy NR, Ju Z, Lechel A, Stepczynska A, Lilli DR, Clark AB, Rudolph C, Kuhnel F, Wei K, Schlegelberger B, Schirmacher P, Kunkel TA, Greenberg RA, Edelmann W, Rudolph KL. Exonuclease-1 deletion impairs DNA damage signaling and prolongs lifespan of telomere-dysfunctional mice. Cell. 2007;130:863–77.PubMedPubMedCentralCrossRefGoogle Scholar
  22. Schaetzlein S, Chahwan R, Avdievich E, Roa S, Wei K, Eoff RL, Sellers RS, Clark AB, Kunkel TA, Scharff MD, Edelmann W. Mammalian Exo1 encodes both structural and catalytic functions that play distinct roles in essential biological processes. Proc Natl Acad Sci U S A. 2013;110:E2470–9.PubMedPubMedCentralCrossRefGoogle Scholar
  23. Szankasi P, Smith GR. A role for exonuclease I from S. pombe in mutation avoidance and mismatch correction. Science. 1995;267:1166–9.PubMedCrossRefGoogle Scholar
  24. Tallis M, Morra R, Barkauskaite E, Ahel I. Poly(ADP-ribosyl)ation in regulation of chromatin structure and the DNA damage response. Chromosoma. 2014;123:79–90.PubMedCrossRefGoogle Scholar
  25. Wei K, Clark AB, Wong E, Kane MF, Mazur DJ, Parris T, Kolas NK, Russell R, Hou Jr H, Kneitz B, Yang G, Kunkel TA, Kolodner RD, Cohen PE, Edelmann W. Inactivation of Exonuclease 1 in mice results in DNA mismatch repair defects, increased cancer susceptibility, and male and female sterility. Genes Dev. 2003;17:603–14.PubMedPubMedCentralCrossRefGoogle Scholar
  26. Williams BR, Mirzoeva OK, Morgan WF, Lin J, Dunnick W, Petrini JH. A murine model of Nijmegen breakage syndrome. Curr Biol. 2002;12:648–53.PubMedCrossRefGoogle Scholar
  27. Wilson 3rd DM, Carney JP, Coleman MA, Adamson AW, Christensen M, Lamerdin JE. Hex1: a new human Rad2 nuclease family member with homology to yeast exonuclease 1. Nucleic Acids Res. 1998;26:3762–8.PubMedPubMedCentralCrossRefGoogle Scholar
  28. Wu P, Takai H, de Lange T. Telomeric 3′ overhangs derive from resection by Exo1 and Apollo and fill-in by POT1b-associated CST. Cell. 2012;150:39–52.PubMedPubMedCentralCrossRefGoogle Scholar
  29. Zhang F, Shi J, Chen SH, Bian C, Yu X. The PIN domain of EXO1 recognizes poly(ADP-ribose) in DNA damage response. Nucleic Acids Res. 2015;43:10782–94.PubMedPubMedCentralCrossRefGoogle Scholar

Copyright information

© Springer International Publishing AG 2018

Authors and Affiliations

  1. 1.Center for Healthy Aging, Department of Cellular and Molecular MedicineUniversity of CopenhagenCopenhagenDenmark