Multilocus Methylation Assays in Epigenetics
The rapid progress in the field of DNA methylation analysis offers unique opportunities to comprehensively analyze the influence of epigenetic regulation on a broad spectrum of biological processes.
A broad range of methods to determine DNA methylation statuses has been developed, but the decision on the test for DNA methylation will depend on the question to be answered and the sample size which should be investigated.
In particular for NGS-based approaches, the running costs are currently high per sample, and bioinformatic pipelines for interpretation have to be improved, but these problems will be circumvented in the near future, and standardized software solutions will become available in the near future.
Keywords5-Hydroxymethyl cytosine DNA methylation Enrichment-restriction enzyme Affinity enrichment Bisulfite conversion Array hybridization Next-generation sequencing MLPA Pyrosequencing
List of Abbreviations
Copy number variation
Combined bisulfite restriction analysis
Differentially methylated region
Multiplex ligation probe-dependent amplification
Methylation-sensitive restriction enzyme PCR
Polymerase chain reaction
Quantitative analysis of methylated alleles
Reduced representation bisulfite sequencing
Sequencing by ligation
Sequencing by synthesis
Ten-eleven translocation methylcytosine dioxygenase
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