Modeling Lipid Metabolism in Yeast
The various pathways and mechanisms behind fatty acid, lipid, and membrane biosynthesis together form a complex network. Moreover, lipid metabolism does not operate in isolation but rather functions in the context of a whole cell, surrounded by all its other metabolic pathways, a situation that results in additional connectivity and complexity. Computational models can aid to provide understanding of these complex networks and to make sense of interactions on a whole cell or genomic scale. In particular, these models have proven to be valuable to answer biotechnological questions, such as how to increase the biosynthesis of fatty acids. This chapter discusses the development and usage of genome-scale metabolic models in the light of lipid biosynthesis. A special focus is placed on baker’s yeast Saccharomyces cerevisiae and the oleaginous yeast Yarrowia lipolytica and the use of genome-scale metabolic models to answer biotechnological questions.
The author would like to acknowledge support from the US Department of Energy, Office of Science, Office of Biological and Environmental Research, Genomic Science program (DE-SC0008744), and the Novo Nordisk Foundation.
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