Experimental Evolution of Novel Regulatory Activities in Response to Hydrocarbons and Related Chemicals
Bacterial transcriptional regulatory proteins that control catabolism of hydrocarbons and related chemicals have evolved (or are actively evolving) toward specifically detecting compounds that signal the presence of growth substrates. Laboratory evolution of the chemical-binding and response properties of sensory regulators has been achieved by a number of different techniques to generate novel derivatives with desired properties. Such manipulated and selected regulatory proteins are increasingly used in artificial genetic circuitry for improved biodegradation systems, biosensor construction, and in assembling regulatory cascades for synthetic biology within a wide range of biotechnological applications.
Research in the Shingler laboratory is supported by the Swedish Research Council (VR-MH 2016-02047) and the JC Kempe and SM Kempe foundation (JCK-1523).
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