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Genetics and Biochemistry of Biphenyl and PCB Biodegradation

  • Loreine Agulló
  • Dietmar H. Pieper
  • Michael SeegerEmail author
Reference work entry
Part of the Handbook of Hydrocarbon and Lipid Microbiology book series (HHLM)

Abstract

Microorganisms are crucial for the removal of polychlorinated biphenyls (PCBs) from polluted environments. Microbial anaerobic dehalogenation of highly and moderately chlorinated biphenyls generates the subsequent less chlorinated congeners. Microbial aerobic degradation performed by enzymes of the biphenyl (bph) upper and lower pathways oxidizes moderately and low chlorinated biphenyls. These enzymes and their substrate specificities are discussed in Sect. 2.1. Biphenyl 2,3-dioxgenases (BDOs) are key enzymes of biphenyl pathways, which determine substrate range and extent of PCB degradation. In addition, the specificity of subsequent enzymes is also crucial for productive metabolism. Specific native and engineered BDOs possess a wide range of substrates, which permit their application for synthesis of fine organic chemicals including novel bioactive compounds. The metabolism of PCBs is described in detail for some model organisms, and the genetic organization of gene clusters of model organisms is described in Sect. 2.2. The sequenced genomes of some PCB-metabolizing organisms including the model strains Burkholderia xenovorans LB400 and Rhodococcus jostii RHA1 improve the understanding of their overall metabolism, physiology, and evolution as described in Sect. 2.3. This has also allowed a better evaluation into genome and proteome-wide defenses against PCB toxicity, which is summarized in Sect. 2.4. However, our knowledge on enzymes and genes involved in PCB metabolism is still rather fragmentary and an overview of the diversity of enzymes reported and mosaic routes is given in Sect. 2.5. Finally, strategies to optimize microorganisms for improved PCB degradation and bioremediation processes are discussed in Sects. 2.6 and 2.7.

Notes

Acknowledgments

M.S. gratefully acknowledges support from the grants FONDECYT (1070507, 1020221, 1110992, 1151174, 7020221, 7070174, 7080148, 7090079, and 7100027), USM (130522, 130836, 130948, 131109, 131342, 131562), MILENIO P04/007-F (MIDEPLAN), and CONICYT-BMBF. D.P. gratefully acknowledges support from the grant EU GOCE 003998 (BIOTOOL) and BACSIN.

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Loreine Agulló
    • 1
  • Dietmar H. Pieper
    • 2
  • Michael Seeger
    • 1
    Email author
  1. 1.Laboratorio de Microbiología Molecular y Biotecnología Ambiental, Department of Chemistry and Center for Nanotechnology and Systems Biology and Centro de BiotecnologíaUniversidad Técnica Federico Santa MaríaValparaísoChile
  2. 2.Microbial Interactions and Processes Research GroupHZI – Helmholtz Centre for Infection ResearchBraunschweigGermany

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