Advances in Lipid Analysis/Lipidomics – Analyses of Phospholipids by Recent Application of Mass Spectrometry
Mass spectrometry (MS) has become a most useful tool in the analysis of phospholipids. Analysis of molecular species of phospholipids adding to that of their classes and subclasses is necessary to elucidate their physiological functions. As analytical methods for lipidomics, basically three different types of approaches in the identification of phospholipid molecular species can be selected. The first one is shotgun LC-MS/MS analysis with data-dependent scan, the second one is structure-related focused methods such as precursor ion scanning or neutral loss scanning. Both types of data can be subjected to our search engine, “Lipid Search” (http://lipidsearch.jp), and most probable molecular species can be obtained with their compensated ion intensities. The lipid database for this search engine was constructed theoretically from their structure similarities and variations in polar head groups and fatty carbonyl chains. And identified individual molecular species can be automatically profiling according to their compensated ion intensities. The third method, such as multiple reaction monitoring, is also important for detecting very small amounts of targeted molecules such as lipid mediators or oxidized lipid metabolites. The choice of these three different kinds of methods seems to be very important for neurochemical research for detecting different kinds of lipid metabolites such as unknown lipid ligands, focused class of lipids, or targeted minor lipid mediators.
KeywordsMolecular Species Multiple Reaction Monitoring Neutral Loss Polar Head Group Fatty Acyl Chain
List of Abbreviations:
high-performance liquid chromatography
ultra performance liquid chromatography
This work was supported by special Coordination fund from the Ministry of Education, Culture, Sports, Science and Technology of the Japanese Government, and a fund from Core Research for Evolutional and Technology (CREST) of Japan Science and Technology Agency (JST).
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