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Engineering Cell and Organ Cultures from Medicinal and Aromatic Plants Toward Commercial Production of Bioactive Metabolites

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Book cover Bioprocessing of Plant In Vitro Systems

Part of the book series: Reference Series in Phytochemistry ((RSP))

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Abstract

Production of secondary metabolites from in vitro cell and hairy root cultures (CHRC) of medicinal and aromatic plants (MAP) is considered a promising alternative to gathering plant material from MAP natural populations, often a reason for their overexploitation and even extinction. However, most of the valuable secondary metabolites extracted from different MAP species are present in very low amounts in the respective CHRC. Plant metabolic engineering offers an attractive opportunity to increase the content of target secondary metabolites in engineered transgenic CHRC for production at feasible levels. Moreover, applying metabolic engineering makes it possible to redirect target metabolic pathway(s) in the transgenic CHRC to produce new compounds not present in the wild plant itself. This chapter describes the strategies and experimental toolbox for plant metabolic engineering with examples from engineering secondary metabolite production in CHRC from MAP, as well as a review of these century reported studies on metabolic engineering of CHRC. The directions and prospects for CHRC metabolic engineering applications in production of valuable secondary metabolites are discussed

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Abbreviations

CHRC:

Cell and hairy root culture

CS:

Cell suspension

HR:

Hairy roots

MAP:

Medicinal and aromatic plants

ME:

Metabolic engineering

RNAi:

RNA interference

TF:

Transcription factor

TIA:

Monoterpenoid indole alkaloids

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Authors and Affiliations

  1. AgroBioInstitute, 8 Dragan Tzankov blvd, Sofia, 1164, Bulgaria

    Krasimir Rusanov & Ivan Atanassov

  2. Joint Genomic Center Ltd., 8 Dragan Tzankov blvd, Sofia, 1164, Bulgaria

    Atanas Atanassov

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  1. Krasimir Rusanov
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  2. Atanas Atanassov
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  3. Ivan Atanassov
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Correspondence to Krasimir Rusanov .

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  1. University of Food Technologies, Plovdiv, Bulgaria

    Atanas Pavlov

  2. Bioprocess Engineering, Institute of Food Technology and Bioprocess Engineering, TU Dresden, Dresden, Germany

    Thomas Bley

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Rusanov, K., Atanassov, A., Atanassov, I. (2018). Engineering Cell and Organ Cultures from Medicinal and Aromatic Plants Toward Commercial Production of Bioactive Metabolites. In: Pavlov, A., Bley, T. (eds) Bioprocessing of Plant In Vitro Systems. Reference Series in Phytochemistry. Springer, Cham. https://doi.org/10.1007/978-3-319-54600-1_8

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