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Biosensors of the Well-being of Cell Cultures

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Handbook of Cell Biosensors

Abstract

Genetically-encoded biosensors offer advantages over traditional analytical technologies in biological manufacturing. Various biosensor architectures can be employed based on activation of transcription, translation, or folding of a reporter. Such biosensors can be used in small volume, high throughput culture devices to provide a more complete picture of cellular physiology. In addition, because of their simple read out, genetically encoded biosensors can be employed for monitoring during production and offer the opportunity for increased measurement density. This chapter reviews current applications of biosensors to measure cellular health and well-being including designs for detecting the activation of cell stress responses as well as sensing of critical metabolites. Many of these designs have been demonstrated to be relevant to bioprocess engineering or cell line selection, but to date very few have been adopted by industry. In future, ensuring that biosensors function robustly under industrial conditions is a priority, as is designing systems that are compatible with industrial workflows. In the long term, engineering systems that move beyond sensing, to include the ability to adjust the environment of the culture based on signals, will allow biotechnology to fully capitalize on the advantages that genetically encoded biosensors can bring.

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Authors and Affiliations

  1. Department of Chemical Engineering, Imperial College Centre for Synthetic Biology, London, UK

    Karen Marie Polizzi

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  1. Karen Marie Polizzi
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Correspondence to Karen Marie Polizzi .

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Editors and Affiliations

  1. UMR CNRS 6144 GEPEA, Université de Nantes, La Roche sur Yon, France

    Gerald Thouand

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  1. Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel

    Shimshon Belkin

  2. Faculty of Medicine, Imperial College, London, UK

    Paul Freemont

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Polizzi, K.M. (2019). Biosensors of the Well-being of Cell Cultures. In: Thouand, G. (eds) Handbook of Cell Biosensors. Springer, Cham. https://doi.org/10.1007/978-3-319-47405-2_119-1

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  • DOI: https://doi.org/10.1007/978-3-319-47405-2_119-1

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