Skip to main content
SpringerLink
Log in

In Situ: Groundwater Bioremediation

  • Living reference work entry
  • First Online:
Consequences of Microbial Interactions with Hydrocarbons, Oils, and Lipids: Biodegradation and Bioremediation

Part of the book series: Handbook of Hydrocarbon and Lipid Microbiology ((HHLM))

Abstract

In situ groundwater bioremediation of hydrocarbons has been used for more than 40 years. Most strategies involve biostimulation; however, recently bioaugmentation have been used for dehalorespiration. Aquifer and contaminant profiles are critical to determining the feasibility and strategy for in situ groundwater bioremediation. Hydraulic conductivity and redox conditions, including concentrations of terminal electron acceptors, are critical to determine the feasibility and strategy for potential bioremediation applications. Conceptual models followed by characterization and subsequent numerical models are critical for efficient and cost-effective bioremediation. Critical research needs in this area include better modeling and integration of remediation strategies with natural attenuation.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution

Institutional subscriptions

References

  • Alfoldi L (1988) Groundwater microbiology, and problems and biological treatment-state-of-the-art report. Water Sci Technol 20:1–31

    Article  CAS  Google Scholar 

  • Baker KH, Herson DS (1990) In situ bioremediation of contaminated aquifers and subsurface soils. Geomicrobiol J 8:133–146

    Article  CAS  Google Scholar 

  • Bekins BA, Godsy EM, Goerlitz DF (1993) Modeling steady-state methanogenic degradation of phenols in groundwater. J Contam Hydrol 14:279–294

    Article  CAS  Google Scholar 

  • Borden RC, Bedient PB (1986) Transport of dissolved hydrocarbons influenced by reaeration and oxygen limited biodegradation 1. Theoretical development. Water Resour Res 22:1973–1982

    Article  CAS  Google Scholar 

  • Chiang CY, Salanitro JP, Chai EY, Colthart JD, Klein CL (1989) Aerobic biodegradation o benzene, toluene, and xylene in sandy aquifer, and data analysis and computer modeling. Ground Water 27:823–834

    Article  CAS  Google Scholar 

  • Choi NC, Choi JW, Kim SB, Park SJ, Kim DJ (2009) Two-dimensional modelling of benzene transport and biodegradation in a laboratory-scale aquifer. Environ Technol 30:53–62

    Article  CAS  PubMed  Google Scholar 

  • Cusack F, Singh S, McCarthy C, Grieco J, Derocco M, Nguyen D, Lappinscott H, Costerton JW (1992) Enhanced oil-recovery – 3-dimensional sandpack simulation of ultramicrobacteria resuscitation in reservoir formation. J Gen Microbiol 138:647–655

    Article  Google Scholar 

  • Deflaun MF, Marshall BM, Kulle EP, Levy SB (1994) Tn5 insertion mutants of Pseudomonas fluorescens defective in adhesion to soil and seeds. Appl Environ Microbiol 60:2637–2642

    PubMed  PubMed Central  CAS  Google Scholar 

  • Enzien MV, Picardal F, Hazen TC, Arnold RG, Fliermans CB (1994) Reductive Dechlorination of trichloroethylene and tetrachloroethylene under aerobic conditions in a sediment column. Appl Environ Microbiol 60:2200–2204

    PubMed  PubMed Central  CAS  Google Scholar 

  • Faybishenko B, Hazen TC, Long PE, Brodie EL, Conrad ME, Hubbard SS, Christensen JN, Joyner D, Borglin SE, Chakraborty R, Williams KH, Peterson JE, Chen JS, Brown ST, Tokunaga TK, Wan JM, Firestone M, Newcomer DR, Resch CT, Cantrell KJ, Willett A, Koenigsberg S (2008) In situ long-term reductive bioimmobilization of Cr(VI) in groundwater using hydrogen release compound. Environ Sci Technol 42:8478–8485

    Article  CAS  PubMed  Google Scholar 

  • Fredrickson JK, Hicks RJ, Li SW, Brockman FJ (1988) Plasmid incidence in bacteria from deep subsurface sediments. Appl Environ Microbiol 54:2916–2923

    PubMed  PubMed Central  CAS  Google Scholar 

  • Ginn TR, Wood BD, Nelson KE, Scheibe TD, Murphy EM, Clement TP (2002) Processes in microbial transport in the natural subsurface. Adv. Water Resour 25:1017–1042

    Article  CAS  Google Scholar 

  • Harvey RW, George LH (1987) Growth determination for unattached bacteria in a contaminated aquifer. Appl Environ Microbiol 53:2992–2996

    PubMed  PubMed Central  CAS  Google Scholar 

  • Harvey RW, Smith RL, George LH (1984) Effect of organic contamination upon microbial distributions and heterotrophic uptake in a cape cod, Mass. Aquifer. Appl Environ Microbiol 48:1197–1202

    PubMed  PubMed Central  CAS  Google Scholar 

  • Hazen TC, Lombard KH, Looney BB, Enzien MV, Dougherty JM, Fliermans CB, Wear J, Eddy-Dilek CA (1994) Summary of in situ bioremediation demonstration (methane biostimulation) via horizontal wells at the Savannah River site integrated demonstration project. In: Gee GW, Wing NR (eds) Proceedings thirty-third Hanford symposium on health and the environment in-situ remediation: scientific basis for current and future technologies. Battelle Press, Columbus, pp 135–150

    Google Scholar 

  • Hazen TC, Jimenez L, De Victoria GL, Fliermans CB (1991) Comparison of bacteria from deep subsurface sediment and adjacent groundwater. Microb Ecol 22:293–304

    Article  CAS  PubMed  Google Scholar 

  • Hazen TC, Sayler GS (2016) Environmental systems microbiology of contaminated environments. In: Yates M, Nakatsu C, Miller R (eds) Manual of environmental microbiology, 4th edn. ASM Press, Washington, DC

    Google Scholar 

  • Hazen TC, Tabak HH (2005) Developments in bioremediation of soils and sediments polluted with metals and radionuclides: 2. Field research on bioremediation of metals and radionuclides. Rev Environ Sci Biotechnol 4:157–183

    Article  CAS  Google Scholar 

  • Hazen TC (1997) Bioremediation. In: Haldeman PA (ed) Microbiology of the terrestrial subsurface. CRC Press, Boca Raton, pp 247–266

    Google Scholar 

  • Horvath RS (1972) Microbial co-metabolism and the degradation of organic compounds in nature. Bacteriol Rev 36:146–155

    PubMed  PubMed Central  CAS  Google Scholar 

  • Hubbard SS, Williams K, Conrad ME, Faybishenko B, Peterson J, Chen JS, Long P, Hazen TC (2008) Geophysical monitoring of hydrological and biogeochemical transformations associated with Cr(VI) bioremediation. Environ Sci Technol 42:3757–3765

    Article  CAS  PubMed  Google Scholar 

  • Johnson WP, Zhang P, Fuller ME, Scheibe TD, Mailloux BJ, Onstott TC, DeFlaun MF, Hubbard SS, Radtke J, Kovacik WP, Holben W (2001) Ferrographic tracking of bacterial transport in the field at the Narrow Channel focus area, Oyster, VA. Environ Sci Technol 35:182–191

    Article  CAS  PubMed  Google Scholar 

  • Keet BA (1995) Bioslurping state of the art. In: Hinchee RE, Kittel JA, Reisinger HJ (eds) Applied bioremediation of petroleum hydrocarbons. Battelle Press, Columbus, pp 329–334

    Google Scholar 

  • Konikow LF, Bredeheoft JD (1978) Computer model of two dimensional solute transport and dispersion in ground water. Automated data processing and computations. Techniques of Water Resources Investigations of the U.S. Geological Survey, Washington, DC

    Google Scholar 

  • Lee ES, Schwartz FW (2007) Characterization and optimization of long-term controlled release system for groundwater remediation: a generalized modeling approach. Chemosphere 69:247–253

    Article  CAS  PubMed  Google Scholar 

  • Litchfield CD (1993) In situ bioremediation: basis and practices. In: Levin MA, Gealt MA (eds) Biotreatment of industrial and hazardous waste. McGraw-Hill, New York, pp 167–196

    Google Scholar 

  • Loffler FE, Sun Q, Li J, Tiedje JM (2000) 16S rRNA gene-based detection of Tetrachloroethene-dechlorinating Desulfuromonas and Dehalococcoides species. Appl Environ Microbiol 66:1369–3445

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Major D, Cox E, Edwards E, Hare PW (1994) The complete dechlorination of trichloroethene to ethene under natural conditions in a shallow bedrock aquifer located in New York state. In: Proceedings of the EPA Symposium on Intrinsic Bioremediation of Ground Water, August 30–September 1, Denver, CO. U.S. Environmental Protection Agency, EPA/540/R-94/515

    Google Scholar 

  • Martin M, Imbrigiotta TE (1994) Contamination of ground water with trichloroethylene at the building 24 site at Picatinny arsenal, New Jersey. In: Proceedings of the EPA symposium on intrinsic bioremediation of ground water, august 30-September 1, Denver, CO. U.S. Environmental Protection Agency, EPA/540/R-94/515

    Google Scholar 

  • Molz FJ, Widdowson MA, Benefield LD (1986) Simulation of microbial growth dynamics coupled to nutrient and oxygen transport in porous media. Water Resour Res 22:1207–1216

    Article  CAS  Google Scholar 

  • National Research Council (U.S.). Water Science and Technology Board (1993) In situ bioremediation: when does it work? National Academy Press, Washington, DC

    Google Scholar 

  • Nelson MJK, Compeau G, Maziarz T, Mahaffey WR (1994) Laboratory treatability testing for assessment of field applicability. In: Flathman PE, Jerger DE, Exner JH (eds) Bioremediation field experience. Lewis Publishers, Boca Raton, pp 59–80

    Google Scholar 

  • Paradis CJ, Jagadamma S, Watson DB, McKay LD, Hazen TC, Park M, Istok JD (2016) In situ mobility of uranium in the presence of nitrate following sulfate-reducing conditions. J Contam Hydrol 187:55–64

    Article  CAS  PubMed  Google Scholar 

  • Paradis CJ, McKay LD, Perfect E, Istok JD, Hazen TC (2018) Push-pull tests for estimating effective porosity: expanded analytical solution and in situ application. Hydrogeol J 26:381–393

    Article  Google Scholar 

  • Paul EA, Clark FG (1989) Soil microbiology and biochemistry. Academic, San Diego

    Google Scholar 

  • Plaza G, Ulfig K, Hazen TC, Brigmon RL (2001) Use of molecular techniques in bioremediation. Acta Microbiol Pol 50:205–218

    PubMed  CAS  Google Scholar 

  • Raymond RL, Jamison VW, Hudson JO (1977) Beneficial stimulation of bacterial activity in groundwater containing petroleum hydrocarbons. Am Inst Chem Eng Symp Ser 73:390–404

    CAS  Google Scholar 

  • Rifai HS, Bedient PB, Borden RC, Haasbeek JF (1987) BIOPLUME II computer model of two-dimensional contaminant transport under the influence of oxygen limited biodegradation in groundwater User’s manual version 1.0 Houston. Rice University, National Center for ground water research

    Google Scholar 

  • Rifai HS, Bedient PB, Wilson JT, Miller KM, Armstrong JM (1988) Biodegradation modeling at an aviation fuel spill. ASCE J Environ Eng 114:1007–1029

    Article  CAS  Google Scholar 

  • Smillie CS, Smith MB, Friedman J, Cordero OX, David LA, Alm EJ (2011) Ecology drives a global network of gene exchange connecting the human microbiome. Nature 480:241–244

    Article  CAS  PubMed  Google Scholar 

  • Smith MB, Rocha AM, Smillie CS, Olesen SW, Paradis C, Wu L, Campbell JH, Fortney JL, Mehlhorn TL, Lowe KA, Earles JE, Phillips J, Techtmann SM, Joyner DC, Elias DA, Bailey KL, Hurt RA Jr, Preheim SP, Sanders MC, Yang J, Mueller MA, Brooks S, Watson DB, Zhang P, He Z, Dubinsky EA, Adams PD, Arkin AP, Fields MW, Zhou J, Alm EJ, Hazen TC (2015) Natural bacterial communities serve as quantitative geochemical biosensors. MBio 6:e00326–e00315

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Thomas JM, Ward CH (1989) In situ biorestoration of organic contaminants in the subsurface. Environ Sci Technol 23:760–766

    Article  CAS  Google Scholar 

  • Thomas JM, Ward CH (1992) Subsurface microbial ecology and bioremediation. J Hazard Mater 32:179–194

    Article  CAS  Google Scholar 

  • Travis BJ, Rosenberg ND (1997) Modeling in situ bioremediation of TCE at Savannah River: effects of product toxicity and microbial interactions on TCE degradation. Environ Sci Technol 31:3093–3102

    Article  CAS  Google Scholar 

  • Tremblay D, Tulis D, Kostecki P, Ewald K (1995) Innovation skyrockets at 50,000 LUST sites, EPA study reveal technology use at LUST sites. Soil Groundwater Cleanup 1995:6–13

    Google Scholar 

  • USEPA (1988) Clean-up of releases from petroleum USTs. Vol. Report-530-UST-88-001 USEPA, Washington, DC

    Google Scholar 

  • USEPA (1989) Bioremediation of hazardous waste sites workshop. CERI-89-11. Washington, DC

    Google Scholar 

  • USEPA (2013) Introduction to in situ bioremediation of groundwater. EPA 542-R-13-018

    Google Scholar 

  • USEPA (2017) How to evaluate alternative cleanup technologies for underground storage tank sites. EPA 510-B-17-003

    Google Scholar 

  • Widdowson MA, Molz FA, Benefield LD (1987) Development and application of a model for simulating microbial growth dynamics coupled to nutrient and oxygen transport in porous media. In: Proceedings of the Association of Groundwater Scientists and Engineers/international ground water model center, pp 28–51, Holcomb research center institute conference on solving ground water problems with models. Dublin: National Ground Water Association

    Google Scholar 

  • Widdowson MA, Molz FJ, Benefield LD (1988) A numerical transport model for oxygen- and nitrate-based respiration linked to substrate and nutrient availability in porous media. Water Resour Res 24:1553–1565

    Article  CAS  Google Scholar 

  • Wilson JT, Weaver JW, Kampbel DH (1994) Intrinsic bioremediation of TCE in ground water at an NPL site in St. Joseph, Michigan. Vol. proceedings of the EPA symposium on intrinsic bioremediation of ground water, august 30-September 1, Denver, CO. U.S. Environmental Protection Agency, EPA/540/R-94/515

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Civil and Environmental Engigeering, Department of Earth and Planetry Sciences, Department of Microbiology, Institute for Secure and Sustainable Environment, Universith of Tennessee, Knoxville, TN, USA

    Terry C. Hazen

  2. Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA

    Terry C. Hazen

Authors
  1. Terry C. Hazen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Terry C. Hazen .

Editor information

Editors and Affiliations

  1. Cape Coral, Florida, USA

    Robert Steffan

Rights and permissions

Reprints and permissions

Copyright information

© 2018 Springer International Publishing AG, part of Springer Nature

About this entry

Check for updates. Verify currency and authenticity via CrossMark

Cite this entry

Hazen, T.C. (2018). In Situ: Groundwater Bioremediation. In: Steffan, R. (eds) Consequences of Microbial Interactions with Hydrocarbons, Oils, and Lipids: Biodegradation and Bioremediation. Handbook of Hydrocarbon and Lipid Microbiology . Springer, Cham. https://doi.org/10.1007/978-3-319-44535-9_11-1

Download citation

  • DOI: https://doi.org/10.1007/978-3-319-44535-9_11-1

  • Received:

  • Accepted:

  • Published:

  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-44535-9

  • Online ISBN: 978-3-319-44535-9

  • eBook Packages: Springer Reference Biomedicine and Life SciencesReference Module Biomedical and Life Sciences

Publish with us

Policies and ethics

Search

Navigation