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Identification of Contact Allergens by In Vitro Cell Culture-Based Methods

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Book cover Kanerva’s Occupational Dermatology

Abstract

If an in vitro model is to replace an animal model, it should perform to at least the same degree of accuracy as current animal models. Most importantly it should correlate to the human experience.

Currently it is thought that no single assay will meet these requirements and that a battery of assays that mimic sensitization in vitro should be developed.

It is thought that such a battery of human cell-based assays will mimic the human situation more than animal cell-based assays.

Assays reflecting epidermal penetration and activation, dendritic cell maturation and migration, and T cell priming assays are being developed.

The irritant property of a sensitizer may be related to sensitizer potency and may enable sensitizer potency to be determined with the aid of an epidermal equivalent assay.

A proteomic approach to investigate the effect of chemical sensitizers on keratinocyte metabolism may identify novel biomarkers.

Genomic and proteomic studies are identifying dendritic cell signatures which identify sensitizers from non-sensitizers.

In vitro T cell priming assays can be used to distinguish sensitizers from non-sensitizers. The nature of these assays, however, makes it currently unlikely that they will be suitable for high-throughput screening.

Progress toward validation is slow but steadily moving forward. A number of assays are being identified which may be suitable for pre-validation studies and then validation studies.

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Authors and Affiliations

  1. Department of Dermatology, VU University Medical Centre, Amsterdam, The Netherlands

    Susan Gibbs

  2. Allergy Research Group, Department of Dermatology, Faculty of Medicine, University of Freiburg, Freiburg, Germany

    Stefan F. Martin

  3. Department of Pharmacological Sciences, Università degli Studi di Milano, Milan, Italy

    Emanuela Corsini

  4. Department of Dermatology, Venereology and Allergology, University Medical Center and Medical Faculty Mannheim, University of Heidelberg, Center of Excellence in Dermatology, Mannheim, Germany

    Hermann-Josef Thierse

  5. Current address: Department of Chemical and Product Safety, German Federal Institute for Risk Assessment, Berlin, Germany

    Hermann-Josef Thierse

Authors
  1. Susan Gibbs
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  2. Stefan F. Martin
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  3. Emanuela Corsini
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  4. Hermann-Josef Thierse
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Corresponding author

Correspondence to Hermann-Josef Thierse .

Editor information

Editors and Affiliations

  1. Department of Dermatology, University of Osnabrück, Osnabrück, Niedersachsen, Germany

    Swen Malte John

  2. Department of Dermato-allergology, Gentofte Hospital, Hellerup, Denmark

    Jeanne Duus Johansen

  3. Dept. of Dermatology and Allergology, VU University Medical Centre, Amsterdam, The Netherlands

    Thomas Rustemeyer

  4. Dept. of Dermatology and Allergology, Friedrich Schiller University, Jena, Thüringen, Germany

    Peter Elsner

  5. Department of Dermatology, University of California San Francisco, San Francisco, California, USA

    Howard I. Maibach

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Gibbs, S., Martin, S.F., Corsini, E., Thierse, HJ. (2018). Identification of Contact Allergens by In Vitro Cell Culture-Based Methods. In: John, S., Johansen, J., Rustemeyer, T., Elsner, P., Maibach, H. (eds) Kanerva’s Occupational Dermatology. Springer, Cham. https://doi.org/10.1007/978-3-319-40221-5_106-2

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  • DOI: https://doi.org/10.1007/978-3-319-40221-5_106-2

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-40221-5

  • Online ISBN: 978-3-319-40221-5

  • eBook Packages: Springer Reference MedicineReference Module Medicine

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