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Cancer Exosomes for Early Pancreatic Cancer Diagnosis and Role in Metastasis

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Pancreatic Cancer

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Abstract

Pancreatic ductal adenocarcinoma (PDAC) is a treatment-recalcitrant and highly metastatic cancer. Recent studies have demonstrated that PDAC is associated with an increased release of small vesicles called exosomes that are ~40 to 130 nanometers in diameter. These exosomes may derive from pancreatic cancer cells, cancer-associated fibroblasts, and infiltrating immune and inflammatory cells. They carry a cargo rich in proteins, lipids, DNA, and microRNAs. Exosomes can modulate the tumor microenvironment, promote pancreatic cancer cell proliferation, invasion, and metastasis, and prime the pre-metastatic niche to facilitate formation of distant metastatic lesions. Components of the exosomal cargo may also serve as diagnostic biomarkers and guide the design of precision medicine strategies. Finally, exosomes have been proposed to act as biological nanoparticles that can be loaded with drugs for therapeutic use.

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References

  1. Kowal J, Tkach M, Théry C. Biogenesis and secretion of exosomes. Curr Opin Cell Biol. 2014;29:116–25.

    Article  CAS  Google Scholar 

  2. Razi M, Futter CE. Distinct Roles for Tsg101 and Hrs in Multivesicular Body Formation and Inward Vesiculation. Mol Biol Cell. 2006;17:3469–83.

    Article  CAS  Google Scholar 

  3. Raposo G, Stoorvogel W. Extracellular vesicles: exosomes, microvesicles, and friends. J Cell Biol. 2013;200:373–83.

    Article  CAS  Google Scholar 

  4. Colombo M, Raposo G, Théry C. Biogenesis, secretion, and intercellular interactions of exosomes and other extracellular vesicles. Annu Rev Cell Dev Biol. 2014;30:255–89.

    Article  CAS  Google Scholar 

  5. Crescitelli R, Lässer C, Szabó T, Kittel A, Eldh M, Dianzani I, et al. Distinct RNA profiles in subpopulations of extracellular vesicles: aapoptotic bodies, micro-vesicles and exosomes. J Extracell Vesicles. 2013;2:20677. https://doi.org/10.3402/jev.v2i0.20677.

    Article  CAS  Google Scholar 

  6. Marsh M, McMahon HT. The structural era of endocytosis. Science. 1999;285:215–20.

    Article  CAS  Google Scholar 

  7. Mellman I. Endocytosis and molecular sorting. Annu Rev Cell Dev Biol. 1996;12:575–625.

    Article  CAS  Google Scholar 

  8. McMahon HT, Boucrot E. Molecular mechanism and physiological functions of clathrin-mediated endocytosis. Nat Rev Mol Cell Biol. 2011;12:517–33.

    Article  CAS  Google Scholar 

  9. Verweij FJ, Middeldorp JM, Pegtel DM. Intracellular signaling controlled by the endosomal-exosomal pathway. Commun Integr Biol. 2012;5:88–93.

    Article  CAS  Google Scholar 

  10. Miaczynska M, Pelkmans L, Zerial M. Not just a sink: endosomes in control of signal transduction. Curr Opin Cell Biol. 2004;16:400–6.

    Article  CAS  Google Scholar 

  11. Sorkin A, Goh LK. Endocytosis and intracellular trafficking of ErbBs. Exp Cell Res. 2009;315:683–96.

    Article  CAS  Google Scholar 

  12. White IJ, Bailey LM, Aghakhani MR, Moss SE, Futter CE. EGF stimulates annexin 1-dependent inward vesiculation in a multivesicular endosome subpopulation. EMBO J. 2006;25:1–12.

    Article  CAS  Google Scholar 

  13. Witwer KW, Buzás E, Bemis LT, Bora A, Lässer C, Lötvall J, et al. Standardization of sample collection, isolation and analysis methods in extracellular vesicle research. J Extracell Vesicles. 2013;2:20360. https://doi.org/10.3402/jev.v2i0.20360.

    Article  CAS  Google Scholar 

  14. Greening DW, Xu R, Ji H, Tauro BJ, Simpson RJ. A protocol for exosome isolation and characterization: Evaluation of ultracentrifugation, density-gradient separation, and immunoaffinity capture methods. Methods Mol Biol. 2015;1295:179–209.

    Article  CAS  Google Scholar 

  15. Li P, Kaslan M, Lee SH, Yao J, Gao Z. Progress in Exosome Isolation Techniques. Theranostics. 2017;7:789–804.

    Article  CAS  Google Scholar 

  16. Nakai W, Yoshida T, Diez D, Miyatake Y, Nishibu T, Imawaka N, Naruse K, et al. A novel affinity-based method for the isolation of highly purified extracellular vesicles. Sci Rep. 2016;6:33935. https://doi.org/10.1038/srep33935.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Denzer K, Kleijmeer MJ, Heijnen HF, Stoorvogel W, Geuze HJ. Exosome: from internal vesicle of the multivesicular body to intercellular signaling device. J Cell Sci. 2000;19:3365–74.

    Google Scholar 

  18. van Niel G, Porto-Carreiro I, Simoes S, Raposo G. Exosomes: a common pathway for a specialized function. J Biochem. 2006;140:13–21.

    Article  Google Scholar 

  19. Schey KL, Luther JM, Rose KL. Proteomics charactherization of exosome cargo. Methods. 2015;87:75–82.

    Article  CAS  Google Scholar 

  20. López-Cobo S, Campos-Silva C, Valés-Gómez M. Glycosyl-Phosphatidyl-Inositol (GPI)-Anchors and Metalloproteases: Their Roles in the Regulation of Exosome Composition and NKG2D-Mediated Immune Recognition. Front Cell Dev Biol. 2016;4:97. https://doi.org/10.3389/fcell.2016.00097.

    Article  PubMed  PubMed Central  Google Scholar 

  21. Liu W, Litwack ED, Stanley MJ, Langford JK, Lander AD, Sanderson RD. Heparan sulfate proteoglycans as adhesive and anti-invasive molecules. Syndecans and glypican have distinct functions. J Biol Chem. 1998;273:22825–32.

    Article  CAS  Google Scholar 

  22. Kleeff J, Ishiwata T, Kumbasar A, Friess H, Büchler MW, Lander AD, et al. The cell-surface heparan sulfate proteoglycan glypican-1 regulates growth factor action in pancreatic carcinoma cells and is overexpressed in human pancreatic cancer. J Clin Invest. 1998;102:1662–73.

    Article  CAS  Google Scholar 

  23. Aikawa T, Whipple CA, Lopez ME, Gunn J, Young A, Lander AD, et al. Glypican-1 modulates the angiogenic and metastatic potential of human and mouse cancer cells. J Clin Invest. 2008;118:89–99.

    Article  CAS  Google Scholar 

  24. Whipple CA, Young AL, Korc M. A KrasG12D-driven genetic mouse model of pancreatic cancer requires glypican-1 for efficient proliferation and angiogenesis. Oncogene. 2012;31:2535–44.

    Article  CAS  Google Scholar 

  25. Häcker U, Nybakken K, Perrimon N. Heparan sulphate proteoglycans: the sweet side of development. Nat Rev Mol Cell Biol. 2005;6:530–41.

    Article  Google Scholar 

  26. Rodgers KD, San Antonio JD, Jacenko O. Heparan sulfate proteoglycans: a GAGgle of skeletal-hematopoietic regulators. Dev Dyn. 2008;237:2622–42.

    Article  CAS  Google Scholar 

  27. Christianson HC, Svensson KJ, van Kuppevelt TH, Li JP, Belting M. Cancer cell exosomes depend on cell-surface heparan sulfate proteoglycans for their internalization and functional activity. Proc Natl Acad Sci USA. 2013;110:17380–5.

    Article  CAS  Google Scholar 

  28. Lai X, Wang M, McElyea SD, Sherman S, House M, Korc M. A microRNA signature in circulating exosomes is superior to exosomal glypican-1 levels for diagnosing pancreatic cancer. Cancer Lett. 2017;393:86–93.

    Article  CAS  Google Scholar 

  29. Théry C, Amigorena S, Raposo G, Clayton A. Isolation and characterization of exosomes from cell culture supernatants and biological fluids. Curr Protoc Cell Biol. 2006;Chapter 3:Unit 3.22. https://doi.org/10.1002/0471143030.cb0322s30.

    Article  PubMed  Google Scholar 

  30. van der Pol E, Coumans FA, Grootemaat AE, Gardiner C, Sargent IL, Harrison P, et al. Particle size distribution of exosomes and microvesicles determined by transmission electron microscopy, flow cytometry, nanoparticle tracking analysis, and resistive pulse sensing. J Thromb Haemost. 2014;12:1182–92.

    Article  Google Scholar 

  31. Momen-Heravi F, Balaj L, Alian S, Tigges J, Toxavidis V, Ericsson M, et al. Alternative methods for characterization of extracellular vesicles. Front Physiol. 2012;3:354. https://doi.org/10.3389/fphys.2012.00354. eCollection 2012

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  32. Gardiner C, Ferreira YJ, Dragovic RA, Redman CW, Sargent IL. Extracellular vesicle sizing and enumeration by nanoparticle tracking analysis. J Extracell Vesicles. 2013;2:19671. https://doi.org/10.3402/jev.v2i0.19671. eCollection 2013.

    Article  CAS  Google Scholar 

  33. Coumans FA, van der Pol E, Böing AN, Hajji N, Sturk G, van Leeuwen TG, et al. Reproducing extracellular vesicle size and concentration determination with tunable resistive pulse sensing. J Extracell Vesicles. 2014;3:25922. https://doi.org/10.3402/jev.v3.25922.

    Article  PubMed  Google Scholar 

  34. Lau C, Kim Y, Chia D, Spielmann N, Eibl G, Elashoff D, et al. Role of pancreatic cancer-derived exosomes in salivary biomarker development. J Biol Chem. 2013;288(37):26888–97.

    Article  CAS  Google Scholar 

  35. Hildonen S, Skarpen E, Halvorsen TG, Reubsaet L. Isolation and mass spectrometry analysis of urinary extraexosomal proteins. Sci Rep. 2016;6:36331. https://doi.org/10.1038/srep36331.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  36. Bala S, Csak T, Momen-Heravi F, Lippai D, Kodys K, Catalano D, et al. Biodistribution and function of extracellular miRNA-155 in mice. Sci Rep. 2015;5:10721. https://doi.org/10.1038/srep10721.

    Article  PubMed  PubMed Central  Google Scholar 

  37. Morishita M, Takahashi Y, Nishikawa M, Sano K, Kato K, Yamashita T, et al. Quantitative analysis of tissue distribution of the B16BL6-derived exosomes using a streptavidin-lactadherin fusion protein and iodine-125-labeled biotin derivative after intravenous injection in mice. J Pharm Sci. 2015;104:705–13.

    Article  CAS  Google Scholar 

  38. Kahlert C, Melo SA, Protopopov A, Tang J, Seth S, Koch M, et al. Identification of double-stranded genomic DNA spanning all chromosomes with mutated KRAS and p53 DNA in the serum exosomes of patients with pancreatic cancer. J Biol Chem. 2014;289:3869–75.

    Article  CAS  Google Scholar 

  39. Madhavan B, Yue S, Galli U, Rana S, Gross W, Müller M, et al. Combined evaluation of a panel of protein and miRNA serum-exosome biomarkers for pancreatic cancer diagnosis increases sensitivity and specificity. Int J Cancer. 2015;136:2616–27.

    Article  CAS  Google Scholar 

  40. Yang S, Che SP, Kurywchak P, Tavormina JL, Gansmo LB, Correa de Sampaio P, et al. Detection of mutant KRAS and TP53 DNA in circulating exosomes from healthy individuals and patients with pancreatic cancer. Cancer Biol Ther. 2017;18:158–65.

    Article  CAS  Google Scholar 

  41. Babic A, Wolpin BM. Circulating Exosomes in Pancreatic Cancer: Will They Succeed on the Long, Littered Road to Early Detection Marker? Clin Chem. 2016;62:307–9.

    Article  CAS  Google Scholar 

  42. Melo SA, Luecke LB, Kahlert C, Fernandez AF, Gammon ST, Kaye J, et al. Glypican-1 identifies cancer exosomes and detects early pancreatic cancer. Nature. 2015;523:177–82.

    Article  CAS  Google Scholar 

  43. Javeed N, Sagar G, Dutta SK, Smyrk TC, Lau JS, Bhattacharya S, et al. Pancreatic Cancer-Derived Exosomes Cause Paraneoplastic β-cell Dysfunction. Clin Cancer Res. 2015;21:1722–33.

    Article  CAS  Google Scholar 

  44. Liang K, Liu F, Fan J, Sun D, Liu C, Lyon CJ, et al. Nanoplasmonic quantification of tumour-derived extracellular vesicles in plasma microsamples for diagnosis and treatment monitoring. Nature Biomed Engineering. 2017; https://doi.org/10.1038/s41551-016-0021.

  45. Fabbri M, Paone A, Calore F, Galli R, Gaudio E, Santhanam R, et al. MicroRNAs bind to Toll-like receptors to induce prometastatic inflammatory response. Proc Natl Acad Sci USA. 2012;109:E2110–6.

    Article  CAS  Google Scholar 

  46. Fonseca P, Vardaki I, Occhionero A, Panaretakis T. Metabolic and Signaling Functions of Cancer Cell-Derived Extracellular Vesicles. Int Rev Cell Mol Biol. 2016;326:175–99.

    Article  CAS  Google Scholar 

  47. Zhao H, Yang L, Baddour J, Achreja A, Bernard V, Moss T, et al. Tumor microenvironment derived exosomes pleiotropically modulate cancer cell metabolism. Elife. 2016;5:e10250. https://doi.org/10.7554/eLife.10250.

    Article  PubMed  PubMed Central  Google Scholar 

  48. Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, et al. Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Mol Cell Proteomics. 2013;12:343–55.

    Article  Google Scholar 

  49. Higginbotham JN, Zhang Q, Jeppesen DK, Scott AM, Manning HC, Ochieng J, et al. Identification and characterization of EGF receptor in individual exosomes by fluorescence-activated vesicle sorting. J Extracell Vesicles. 2016;5:29254. https://doi.org/10.3402/jev.v5.29254. eCollection 2016

    Article  CAS  PubMed  Google Scholar 

  50. Higginbotham JN, Demory Beckler M, Gephart JD, Franklin JL, Bogatcheva G, et al. Amphiregulin exosomes increase cancer cell invasion. Curr Biol. 2011;21:779–86.

    Article  CAS  Google Scholar 

  51. Ene-Obong A, Clear AJ, Watt J, Wang J, Fatah R, Riches JC, et al. Activated pancreatic stellate cells sequester CD8+ T cells to reduce their infiltration of the juxtatumoral compartment of pancreatic ductal adenocarcinoma. Gastroenterology. 2013;145:1121–32.

    Article  CAS  Google Scholar 

  52. Roma-Rodrigues C, Fernandes AR, Baptista PV. Exosome in tumour microenvironment: overview of the crosstalk between normal and cancer cells. Biomed Res Int. 2014;2014:1. https://doi.org/10.1155/2014/179486.

    Article  CAS  Google Scholar 

  53. Mu W, Rana S, Zöller M. Host matrix modulation by tumor exosomes promotes motility and invasiveness. Neoplasia. 2013;15:875–IN4.

    Article  Google Scholar 

  54. Andersen DK, Korc M, Petersen GM, Eibl G, Li D, Rickels MR, Chari ST, Abbruzzese JL. Diabetes Pancreatogenic Diabetes, and Pancreatic Cancer. Diabetes. 2017;66:1103–10.

    Article  CAS  Google Scholar 

  55. Korc M. Pancreatic cancer-associated diabetes is an “exosomopathy”. Clin Cancer Res. 2015;21:1508–10.

    Article  CAS  Google Scholar 

  56. Talbert EE, Guttridge DC. Impaired regeneration: A role for the muscle microenvironment in cancer cachexia. Semin Cell Dev Biol. 2016;54:82–91.

    Article  Google Scholar 

  57. He WA, Calore F, Londhe P, Canella A, Guttridge DC, Croce CM. Microvesicles containing miRNAs promote muscle cell death in cancer cachexia via TLR7. Proc Natl Acad Sci USA. 2014;111:4525–9.

    Article  CAS  Google Scholar 

  58. He WA, Berardi E, Cardillo VM, Acharyya S, Aulino P, Thomas-Ahner J, et al. NF-κB-mediated Pax7 dysregulation in the muscle microenvironment promotes cancer cachexia. J Clin Invest. 2013;123:4821–35.

    Article  CAS  Google Scholar 

  59. Khorana AA, Francis CW, Menzies KE, Wang JG, Hyrien O, Hathcock J, Mackman N, Taubman MB. Plasma tissue factor may be predictive of venous thromboembolism in pancreatic cancer. Journal of Thrombosis and Haemostasis. 2008;6:1983–5.

    Article  CAS  Google Scholar 

  60. Wang JG, Geddings JE, Aleman MM, Cardenas JC, Chantrathammachart P, Williams JC, Kirchhofer D, Bogdanov VY, Bach RR, Rak J, Church FC, Wolberg AS, Pawlinski R, et al. Tumor-derived tissue factor activates coagulation and enhances thrombosis in a mouse xenograft model of human pancreatic cancer. Blood. 2012;119:5543–52.

    Article  CAS  Google Scholar 

  61. Yates KR, Welsh J, Echrish HH, Greenman J, Maraveyas A, Madden LA. Pancreatic cancer cell and microparticle procoagulant surface characterization: involvement of membrane-expressed tissue factor, phosphatidylserine and phosphatidylethanolamine. Blood coagulation & brinolysis. 2011;22:680–7.

    Article  CAS  Google Scholar 

  62. Muhsin-Sharafaldine MR, Kennedy BR, Saunderson SC, Buchanan CR, Dunn AC, Faed JM, et al. Mechanistic insight into the procoagulant activity of tumor-derived apoptotic vesicles. Biochim Biophys Acta. 1861;2017:286–95.

    Google Scholar 

  63. Aharon A, Tamari T, Brenner B. Monocyte-derived microparticles and exosomes induce procoagulant and apoptotic effects on endothelial cells. Thromb Haemost. 2008;100:878–85.

    Article  CAS  Google Scholar 

  64. Khorana AA, Francis CW, Culakova E, Kuderer NM, Lyman GH. Thromboembolism is a leading cause of death in cancer patients receiving outpatient chemotherapy. Journal of Thrombosis and Haemostasis. 2007;5:632–4.

    Article  CAS  Google Scholar 

  65. Chiang AC, Massague J. Molecular basis of metastasis. The New England Journal of Medicine. 2008;359:2814–23.

    Article  CAS  Google Scholar 

  66. Sethi N, Kang Y. Unravelling the complexity of metastasis - molecular understanding and targeted therapies. Nature reviews Cancer. 2011;11:735–48.

    Article  CAS  Google Scholar 

  67. Joyce JA, Pollard JW. Microenvironmental regulation of metastasis. Nature Reviews Cancer. 2009;9:239–52.

    Article  CAS  Google Scholar 

  68. Esquela-Kerscher A, Slack FJ. Oncomirs - microRNAs with a role in cancer. Nature Reviews Cancer. 2006;6:259–69.

    Article  CAS  Google Scholar 

  69. Iorio MV, Croce CM. MicroRNAs in cancer: Small molecules with a huge impact. Journal of Clinical Oncology. 2009;27:5848–56.

    Article  CAS  Google Scholar 

  70. Ma L, Teruya-Feldstein J, Weinberg RA. Tumour invasion and metastasis initiated by microRNA-10b in breast cancer. Nature. 2007;449:682–8.

    Article  CAS  Google Scholar 

  71. Ma L, Reinhardt F, Pan E, Soutschek J, Bhat B, Marcusson EG, Teruya-Feldstein J, Bell GW, Weinberg RA. Therapeutic silencing of miR-10b inhibits metastasis in a mouse mammary tumor model. Nature Biotechnology. 2010;28:341–7.

    Article  CAS  Google Scholar 

  72. Miao F, Zhu J, Chen Y, Tang N, Wang X, Li X. MicroRNA-183-5p promotes the proliferation, invasion and metastasis of human pancreatic adenocarcinoma cells. Oncol Lett. 2016;11:134–40.

    Article  CAS  Google Scholar 

  73. Zhao S, Sun H, Jiang W, Mi Y, Zhang D, Wen Y, et al. miR-4775 promotes colorectal cancer invasion and metastasis via the Smad7/TGFβ-mediated epithelial to mesenchymal transition. Mol Cancer. 2017;16(1):12. https://doi.org/10.1186/s12943-017-0585-z.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  74. Rana S, Malinowska K, Zöller M. Exosomal tumor microRNA modulates premetastatic organ cells. Neoplasia. 2013;15:281–IN31.

    Article  CAS  Google Scholar 

  75. Basso D, Gnatta E, Plebani M. Pancreatic cancer fostered immunosuppression privileges tumor growth and progression. J Clin Cell Immunol. 2014;5:6–22.

    Article  Google Scholar 

  76. Costa-Silva B, Aiello NM, Ocean AJ, Singh S, Zhang H, Thakur BK, et al. Pancreatic cancer exosomes initiate pre-metastatic niche formation in the liver. Nature Cell Biology. 2015;17:816–26.

    Article  CAS  Google Scholar 

  77. Zhang Y, Wang XF. A niche role for cancer exosomes in metastasis. Nature Cell Biology. 2015;17:709–11.

    Article  CAS  Google Scholar 

  78. Friess H, Yamanaka Y, Büchler M, Ebert M, Beger HG, Gold LI, Korc M. Enhanced expression of transforming growth factor beta isoforms in pancreatic cancer correlates with decreased survival. Gastroenterology. 1993;105:1846–56.

    Article  CAS  Google Scholar 

  79. Alvarez-Erviti L, Seow Y, Yin H, Betts C, Lakhal S, Wood MJ. Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes. Nat Biotechnol. 2011;29:341–5.

    Article  CAS  Google Scholar 

  80. Ohno SI, Takanashi M, Sudo K, Ueda S, Ishikawa A, Matsuyama N, et al. Systemically Injected Exosomes Targeted to EGFR Deliver Antitumor MicroRNA to Breast Cancer Cells. Mol Ther. 2013;21:185–91.

    Article  CAS  Google Scholar 

  81. Johnsen KB, Gudbergsson JM, Skov MN, Pilgaard L, Moos T, Duroux M. A comprehensive overview of exosomes as drug delivery vehicles - endogenous nanocarriers for targeted cancer therapy. Biochim Biophys Acta. 1846;2014:75–87.

    Google Scholar 

  82. Kooijmans SA, Aleza CG, Roffler SR, van Solinge WW, Vader P, Schiffelers RM. Display of GPI-anchored anti-EGFR nanobodies on extracellular vesicles promotes tumour cell targeting. J Extracell Vesicles. 2016;5:31053. https://doi.org/10.3402/jev.v5.31053.

    Article  CAS  PubMed  Google Scholar 

  83. Kamerkar S, LeBleu VS, Sugimoto H, Yang S, Ruivo CF, Melo SA, Lee JJ, Kalluri R. Exosomes facilitate therapeutic targeting of oncogenic KRAS in pancreatic cancer. Nature. 2017;546:498–503.

    CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Departments of Medicine, Biochemistry and Molecular Biology, Indiana University School of Medicine, the Melvin and Bren Simon Cancer Center and the Pancreatic Cancer Signature Center, Indianapolis, IN, USA

    Murray Korc

  2. Department of Medicine, Indiana University Melvin and Bren Simon Cancer Center, 980 W. Walnut Street, Indianapolis, IN, 46202, USA

    Samantha Deitz McElyea

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  1. Murray Korc
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  2. Samantha Deitz McElyea
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Correspondence to Murray Korc .

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Editors and Affiliations

  1. Department of General, Visceral and Transplantation Surgery, University of Heidelberg, Heidelberg, Germany

    John P. Neoptolemos

  2. Division of Research Department of Surgery and Genomic Sciences and Precision Medicine Center (GSPMC), Medical College of Wisconsin, Milwaukee, Wisconsin, USA

    Raul Urrutia

  3. Division of Medical Oncology, Duke Cancer Institute, Duke University Medical Center, Durham, North Carolina, USA

    James L. Abbruzzese

  4. Department of General, Visceral and Transplantation Surgery, University of Heidelberg, Heidelberg, Germany

    Markus W. Büchler

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Korc, M., McElyea, S.D. (2018). Cancer Exosomes for Early Pancreatic Cancer Diagnosis and Role in Metastasis. In: Neoptolemos, J., Urrutia, R., Abbruzzese, J., Büchler, M. (eds) Pancreatic Cancer. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-7193-0_67

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