Abstract
This chapter describes a number of aspects important for calibration of matrix-assisted laser desorption/ionization time-of-flight spectra prior to peptide mass fingerprinting searches. Both multipoint internal calibration and mass defect-based calibration is illustrated. The chapter describes how potential internal calibrants, like tryptic autodigest peptides and keratin-related peptides, can be identified and used for high-precision calibration. Furthermore, the construction of project/user-specific lists of potential calibrants is illustrated.
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© 2007 Humana Press Inc., Totowa, NJ
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Hjernø, K., Højrup, P. (2007). Calibration of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Peptide Mass Fingerprinting Spectra. In: Matthiesen, R. (eds) Mass Spectrometry Data Analysis in Proteomics. Methods in Molecular Biology, vol 367. Humana Press. https://doi.org/10.1385/1-59745-275-0:49
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DOI: https://doi.org/10.1385/1-59745-275-0:49
Publisher Name: Humana Press
Print ISBN: 978-1-58829-563-7
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