Abstract
The catalytic subunit of PP2A (PP2Ac) can be purified in milligram quantities from bovine heart using ethanol precipitation, ammonium sulfate precipitation, ion exchange and size exclusion chromatography. The detailed procedure is described to purify PP2Ac over 4 d.
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Tran, H.T., Ferrar, T.S., Ulke-Lemée, A., Moorhead, G.B.G. (2007). Purification of PP2Ac from Bovine Heart. In: Moorhead, G. (eds) Protein Phosphatase Protocols. Methods in Molecular Biology, vol 365. Springer, Totowa, NJ. https://doi.org/10.1385/1-59745-267-X:127
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DOI: https://doi.org/10.1385/1-59745-267-X:127
Publisher Name: Springer, Totowa, NJ
Print ISBN: 978-1-58829-711-2
Online ISBN: 978-1-59745-267-0
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