Synthesis and Use of the Protein Phosphatase Affinity Matrices Microcystin-Sepharose and Microcystin-Biotin-Sepharose

  • Greg B. G. Moorhead
  • Timothy A. J. Haystead
  • Carol MacKintosh
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 365)

Abstract

Microcystin-based affinity matrices have been utilized to demonstrate the association of signaling proteins with protein phosphatases and for the purification of low-abundance microcystin-sensitive protein phosphatases. Here, we describe the procedure for the synthesis and use of microcystin-Sepharose and microcystin-biotin-Sepharose.

Key Words

Microcystin okadaic acid protein phosphatase biotin affinity chromatography cyclic-peptide hepatotoxin cyanobacteria 

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Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  • Greg B. G. Moorhead
    • 1
  • Timothy A. J. Haystead
    • 2
  • Carol MacKintosh
    • 3
  1. 1.Department of Biological SciencesUniversity of CalgaryCalgaryCanada
  2. 2.Department of Pharmacology and Cancer BiologyDuke University Medical CenterDurhamUSA
  3. 3.MRC Protein Phosphorylation Unit, School of Life SciencesUniversity of DundeeDundeeScotland

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