Purification of PP2Ac from Bovine Heart

  • Hue T. Tran
  • Tony S. Ferrar
  • Anne Ulke-Lemée
  • Greg B. G. Moorhead
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 365)

Abstract

The catalytic subunit of PP2A (PP2Ac) can be purified in milligram quantities from bovine heart using ethanol precipitation, ammonium sulfate precipitation, ion exchange and size exclusion chromatography. The detailed procedure is described to purify PP2Ac over 4 d.

Key Words

Protein phosphatase PP2A dephosphorylation column chromatography PP1 bovine heart catalytic subunit 

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Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  • Hue T. Tran
    • 1
  • Tony S. Ferrar
    • 1
  • Anne Ulke-Lemée
    • 1
  • Greg B. G. Moorhead
    • 1
  1. 1.Department of Biological SciencesUniversity of CalgaryCalgaryCanada

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