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Introduction to Macromolecular Refinement

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Macromolecular Crystallography Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 364))

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Abstract

The process of refinement is such a large problem in function minimization that even the computers of today cannot perform the calculations to properly fi X-ray diffraction data. Each of the refinement packages currently under development reduces the difficulty of this problem by utilizing a unique combination of targets, assumptions, and optimization methods. This chapter summarizes the basic methods and underlying assumptions in the commonly used refinement packages. This information can guide the selection of a refinement package that is best suited for a particular refinement project.

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Author information

Authors and Affiliations

  1. Institute of Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, OR

    Dale E. Tronrud

Authors
  1. Dale E. Tronrud
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Editor information

Editors and Affiliations

  1. Department of Microbiology and Molecular Genetics, University of Vermont, Burlingon, VT

    Sylvie Doublié

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© 2007 Humana Press Inc.

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Tronrud, D.E. (2007). Introduction to Macromolecular Refinement. In: Doublié, S. (eds) Macromolecular Crystallography Protocols. Methods in Molecular Biology™, vol 364. Humana Press. https://doi.org/10.1385/1-59745-266-1:231

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  • DOI: https://doi.org/10.1385/1-59745-266-1:231

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-902-4

  • Online ISBN: 978-1-59745-266-3

  • eBook Packages: Springer Protocols

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