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Visible and Fluorescent Staining of Two-Dimensional Gels

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Plant Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 355))

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Abstract

Staining of two-dimensional gel constitutes a crucial step in comparative proteome analysis with respect to the number of proteins analyzed, the accuracy of spot quantification, and compatibility with mass spectrometry. The present chapter describes procedures for several visible and fluorescent dyes compatible with mass spectrometry: colloidal Coomassie blue, silver nitrate, Sypro Ruby®, Deep Purple®, and 5-hexadecanoylamino-fluorescein.

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Author information

Authors and Affiliations

  1. Laboratoire de Protéomique, INRA, Montpellier, France

    François Chevalier, Valérie Rofidal & Michel Rossignol

Authors
  1. François Chevalier
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  2. Valérie Rofidal
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  3. Michel Rossignol
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Editor information

Editors and Affiliations

  1. UMR de Génétique Végétale, La Ferme du Moulon, Gif-sur-Yvette, France

    Hervé Thiellement , Michel Zivy  & Catherine Damerval ,  & 

  2. UMR de Chimie Biologique, Thiverval Grignon, France

    Valérie Méchin

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© 2007 Humana Press Inc., Totowa, NJ

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Chevalier, F., Rofidal, V., Rossignol, M. (2007). Visible and Fluorescent Staining of Two-Dimensional Gels. In: Thiellement, H., Zivy, M., Damerval, C., Méchin, V. (eds) Plant Proteomics. Methods in Molecular Biology, vol 355. Humana Press. https://doi.org/10.1385/1-59745-227-0:145

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  • DOI: https://doi.org/10.1385/1-59745-227-0:145

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-635-1

  • Online ISBN: 978-1-59745-227-4

  • eBook Packages: Springer Protocols

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