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Protein Extraction from Woody Plants

  • Christophe Plomion
  • Céline Lalanne
Part of the Methods in Molecular Biology book series (MIMB, volume 355)

Abstract

In this chapter we present a protocol for total protein extraction optimized for wood-forming tissue (differentiating secondary xylem). The protocol is then used for a series of other organs (root, leaf, pollen, bud, flower, cambium, and phloem) in broadleaf (oak and poplar) and conifer (pine) species. Proteins are first extracted from tissue powdered in liquid nitrogen using the TCA-acetone method and then solubilized in an optimized buffer. The resulting 2D gels can be viewed at http://cbi.labri.fr/outils/protic/index.php.

Key Words

Total protein extraction two-dimensional polyacrylamide gel electrophoresis wood tree 

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Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • Christophe Plomion
    • 1
  • Céline Lalanne
    • 2
  1. 1.Equipe de GénétiqueNRA, UMR BIOGECOCestasFrance
  2. 2.Equipe de GénétiqueINRA, UMR BIOGECOCesta CédexFrance

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