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Fluorescent Proteins and Engineered Cell Lines

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Book cover High Content Screening

Part of the book series: Methods in Molecular Biology ((MIMB,volume 356))

Abstract

Green fluorescent protein and other fluorescent proteins provide powerful tools for high content analysis of cellular processes. Engineering fluorescent protein sensors for expression in cellular assays requires consideration of a wide range of design factors to produce fusion proteins capable of generating informative and biologically relevant data while meeting the rigorous demands of high content screening. The target protein, fluorescent protein, host cell line, construct components and orientation, expression level, and other factors all contribute to the performance of the sensor. This chapter reviews the process of selecting an optimum combination of these design elements for engineering cells for high content analysis.

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Authors and Affiliations

  1. GE Healthcare, The Maynard Centre, Cardiff, UK

    Nick Thomas

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  1. Nick Thomas
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Editors and Affiliations

  1. Cellumen, Inc., Pittsburgh, PA

    D. Lansing Taylor  & Kenneth A. Giuliano  & 

  2. Cellomics, Inc., Pittsburgh, PA

    Jeffrey R. Haskins

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© 2007 Humana Press, Inc.

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Thomas, N. (2007). Fluorescent Proteins and Engineered Cell Lines. In: Taylor, D.L., Haskins, J.R., Giuliano, K.A. (eds) High Content Screening. Methods in Molecular Biology, vol 356. Humana Press. https://doi.org/10.1385/1-59745-217-3:165

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  • DOI: https://doi.org/10.1385/1-59745-217-3:165

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-731-0

  • Online ISBN: 978-1-59745-217-5

  • eBook Packages: Springer Protocols

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