Physiological Indicators of Cell Function

  • Michael J. Ignatius
  • Jeffrey T. Hung
Part of the Methods in Molecular Biology book series (MIMB, volume 356)


Successful high content screening (HCS) assays place large demands on the cell-based reagents used in their development and deployment. Fortunately, there is a wide range of fluorescent physiological indicators from which to choose that are continually increasing in size and variety. Ideal fluorescent reagents for cell-based assays exhibit optimal selectivity, signal intensity, and cell solubility, yet will be easily incorporated into assays across multiple detection platforms. The repertoire of existing fluorogenic and color changing dyes that indicate physiological changes in cells for live cell kinetic and fixed end-point assays are surveyed as well as newly developed reagents for the next generation of HCS assays.

Key Words

Apoptosis assays calcium cell counts cell biology drug discovery expression reporters fluorescence high content screening high content analysis HTS high-throughput screening imaging membrane voltage microplate organelle pathway analysis 


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Copyright information

© Humana Press, Inc. 2007

Authors and Affiliations

  • Michael J. Ignatius
    • 1
  • Jeffrey T. Hung
    • 1
  1. 1.Molecular Probes/InvitrogenEugene

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