Abstract
Proteomic analysis of large numbers of proteins is assisted if each protein species is present at approximately equal concentrations. As such, the extraction of proteins from tissue samples should be designed to maintain a limited dynamic range in the concentration of proteins present. However, in many tissue extracts a high concentration of serum albumin exists from tissue perfusion and/or an inability to effectively rinse the tissue owing to surgical limitations. The analysis of these tissues would be assisted if contaminating serum albumin could be reduced. This chapter outlines a protocol for the effective reduction of serum albumin levels from human myocardium extracts enriched for soluble cytoplasmic proteins.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Corthals, G. L., Wasinger, V. C., Hochstrasser, D. F., and Sanchez, J. C. (2000) The dynamic range of protein expression: a challenge for proteomic research. Electrophoresis 21, 1104–1115.
Rabilloud, T. (1999) Silver staining of 2-D electrophoresis gels. Methods Mol. Biol. 112, 297–305.
Nishihara, J. C. and Champion, K. M. (2002) Quantitative evaluation of proteins in one-and two-dimensional polyacrylamide gels using a fluorescent stain. Electrophoresis 23, 2203–2215.
Hao, Y. (1979) A simple method for the preparation of human serum albumin. Vox Sang. 36, 313–320.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2007 Humana Press Inc.
About this protocol
Cite this protocol
Stanley, B.A., Van Eyk, J.E. (2007). A Method for the Effective Depletion of Albumin From Cellular Extracts. In: Vivanco, F. (eds) Cardiovascular Proteomics. Methods in Molecular Biology™, vol 357. Humana Press. https://doi.org/10.1385/1-59745-214-9:67
Download citation
DOI: https://doi.org/10.1385/1-59745-214-9:67
Publisher Name: Humana Press
Print ISBN: 978-1-58829-535-4
Online ISBN: 978-1-59745-214-4
eBook Packages: Springer Protocols