Abstract
With a mean weight of 1500 g containing around 10 billion neurons, the adult brain represents about 2% of the total body mass, but requires 20% of the total energy produced. It consumes continuously 150 g of glucose and 72 L of oxygen every 24 h. A few minutes interruption of this supply can lead to dramatic brain damage. Manifestations and consequences of stroke depend on the location and extent of the lesions. A vascular cerebral accident, also called stroke or brain attack, is an interruption of the blood supply owing to either occlusion (ischemic stroke) or rupture (hemorrhagic stroke) of a blood vessel to any part of the brain, with an occurrence of around 80% for the ischemic type. Stroke has a devastating impact on public health and remains the third leading cause of death and the first leading cause of long-term disability in industrialized countries. An early diagnosis of the cerebral accident associated with an appropriate treatment would reduce the risk of death and enhance the chances of recovery. When the diagnosis of stroke is established, the physician needs to know the nature (ischemic or hemorrhagic), the extent, and the location of the accident in order to orient patients and to give them most suitable treatment. Because no specific and unique symptoms or early blood diagnostic markers are currently available, it was of a great interest to develop new approaches in the research and discovery area of new early diagnosis and prognosis markers of stroke.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Allard, L., Lescuyer, P., Burgess, J., et al. (2004) ApoC-I and ApoC-III as potential plasmatic markers to distinguish between ischemic and hemorrhagic stroke. Proteomics 4, 2242–2251.
Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.
Blum, H., Beier, H., and Gross, H. J. (1987) Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis 8, 93–99.
Scherl, A., Coute, Y., Deon, C., et al. (2002) Functional proteomic analysis of human nucleolus. Mol. Biol. Cell 13, 4100–4109.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2007 Humana Press Inc.
About this protocol
Cite this protocol
Sanchez, JC., Lescuyer, P., Hochstrasser, D., Allard, L. (2007). Detection of Biomarkers of Stroke Using SELDI-TOF. In: Vivanco, F. (eds) Cardiovascular Proteomics. Methods in Molecular Biology™, vol 357. Humana Press. https://doi.org/10.1385/1-59745-214-9:343
Download citation
DOI: https://doi.org/10.1385/1-59745-214-9:343
Publisher Name: Humana Press
Print ISBN: 978-1-58829-535-4
Online ISBN: 978-1-59745-214-4
eBook Packages: Springer Protocols