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Cardiovascular Proteomics pp 313–318Cite as

Enrichment of Phosphotyrosine Proteome of Human Platelets by Immunoprecipitation

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 357))

Abstract

Proteomics offers the opportunity to comprehensively investigate the anucleate platelet. Here, we present a detailed procedure for enrichment by immunoprecipitation, using the monoclonal antibody 4G10, of the dynamic phosphotyrosine proteome of human platelets. Such an approach offers the possibility of capturing the dynamic tyrosine phosphorylation events that occur upon platelet activation and aggregation, with an aim to identify novel signaling proteins.

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References

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Author information

Authors and Affiliations

  1. Department of Clinical Pharmacology, Royal College of Surgeon’s in Ireland, Dublin, Ireland

    Martina Foy, Donal F. Harney & Kieran Wynne

  2. Department of Molecular Medicine, Conway Institute, University College Dublin, Dublin, Ireland

    Patricia B. Maguire

Authors
  1. Martina Foy
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  2. Donal F. Harney
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  3. Kieran Wynne
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  4. Patricia B. Maguire
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Editor information

Editors and Affiliations

  1. Department of Immunology, Fundación Jiménez Díaz, Universidad Complutense, Madrid, Spain

    Fernando Vivanco

  2. Department of Biochemistry and Molecular Biology I, Universidad Complutense, Madrid, Spain

    Fernando Vivanco

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© 2007 Humana Press Inc.

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Foy, M., Harney, D.F., Wynne, K., Maguire, P.B. (2007). Enrichment of Phosphotyrosine Proteome of Human Platelets by Immunoprecipitation. In: Vivanco, F. (eds) Cardiovascular Proteomics. Methods in Molecular Biology™, vol 357. Humana Press. https://doi.org/10.1385/1-59745-214-9:313

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  • DOI: https://doi.org/10.1385/1-59745-214-9:313

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-535-4

  • Online ISBN: 978-1-59745-214-4

  • eBook Packages: Springer Protocols

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