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Cardiovascular Disease pp 41–67Cite as

Animal Models for Disease

Knockout, Knock-In, and Conditional Mutant Mice

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Part of the book series: Methods in Molecular Medicine ((MIMM,volume 129))

Abstract

Diseases with a genetic basis can be modeled with knockout, knock-in, and conditional mutant gene-targeted mice. In the following, we provide detailed protocols for gene targeting. Gene targeting of embryonic stem cells can be accomplished by laboratories equipped for tissue culture. Alternatively, many gene-targeting services divide the work of targeting with a customer lab. In this collaborative situation, knowledge of the entire process helps ensure a successful outcome. The construction of chimeras for germ-line transmission is not described here, because this procedure is beyond the means of most laboratories, typically is provided by transgenic core facilities, and is best learned through hands-on demonstration.

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Author information

Authors and Affiliations

  1. Department of Genetics, Case Western Reserve University, Cleveland, OH

    David F. LePage & Ronald A. Conlon

Authors
  1. David F. LePage
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  2. Ronald A. Conlon
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Editor information

Editors and Affiliations

  1. Department of Molecular Medicine, Cleveland Clinic Lerner College of Medicine, Case Western Reserve University, Cleveland, OH

    Qing K. Wang PhD, MBA

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© 2006 Humana Press Inc.

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LePage, D.F., Conlon, R.A. (2006). Animal Models for Disease. In: Wang, Q.K. (eds) Cardiovascular Disease. Methods in Molecular Medicine, vol 129. Humana Press. https://doi.org/10.1385/1-59745-213-0:41

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  • DOI: https://doi.org/10.1385/1-59745-213-0:41

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-892-8

  • Online ISBN: 978-1-59745-213-7

  • eBook Packages: Springer Protocols

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