Abstract
To avoid long-double-stranded-RNA-dependent interferon response, short-interfering RNAs (siRNAs) are widely used for RNA interference (RNAi) in mammalian cells. siRNA-based RNAi, however, may not be readily available for the large-scale gene silencing essential for systematic functional genomics, because only a limited fraction of siRNAs is capable of inducing effective mammalian RNAi. siRNAs correctly designed for the knockdown of a particular gene may also destroy the functions of unrelated genes. Here, we describe algorithms by which these serious setbacks can be eliminated in mammalian functional genomics using RNAi and a Web-based online software system for computing highly functional siRNA sequences with maximal target-specificity in mammalian RNAi.
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Ui-Tei, K., Naito, Y., Saigo, K. (2007). Guidelines for the Selection of Effective Short-Interfering RNA Sequences for Functional Genomics. In: Sioud, M. (eds) Target Discovery and Validation Reviews and Protocols. Methods in Molecular Biology™, vol 361. Humana Press. https://doi.org/10.1385/1-59745-208-4:201
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DOI: https://doi.org/10.1385/1-59745-208-4:201
Publisher Name: Humana Press
Print ISBN: 978-1-58829-890-4
Online ISBN: 978-1-59745-208-3
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