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Combinatorial Protein Design Strategies Using Computational Methods

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Protein Engineering Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 352))

Abstract

Computational methods continue to facilitate efforts in protein design. Most of this work has focused on searching sequence space to identify one or a few sequences compatible with a given structure and functionality. Probabilistic computational methods provide information regarding the range of amino acid variability permitted by desired functional and structural constraints. Such methods may be used to guide the construction of both individual sequences and combinatorial libraries of proteins.

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Author information

Authors and Affiliations

  1. Computational Biology Group, Neutron Science Research Center, Quantum Beam Science Directorate, Japan Atomic Energy Agency, Kyoto, Japan

    Hidetoshi Kono

  2. Makineni Theoretical Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, PA

    Wei Wang & Jeffery G. Saven

Authors
  1. Hidetoshi Kono
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  2. Wei Wang
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  3. Jeffery G. Saven
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Editors and Affiliations

  1. Institut für Biologie III, Universität Freiburg, Freiburg, Germany

    Katja M. Arndt  & Kristian M. Müller  & 

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Kono, H., Wang, W., Saven, J.G. (2007). Combinatorial Protein Design Strategies Using Computational Methods. In: Arndt, K.M., Müller, K.M. (eds) Protein Engineering Protocols. Methods in Molecular Biology™, vol 352. Humana Press. https://doi.org/10.1385/1-59745-187-8:3

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  • DOI: https://doi.org/10.1385/1-59745-187-8:3

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-072-4

  • Online ISBN: 978-1-59745-187-1

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