Abstract
The purpose of this chapter is to present basic strategies for the construction of DNA vaccines. This chapter discusses considerations relevant to the selection of a target gene, construction of a DNA expression vector for use as a vaccine, and molecular modifications of the vector to improve protein expression and to augment immunogenicity.
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References
Donnelly, J. J., Martinez, D., Jansen, K. U., Ellis, R. W., Montgomery, D. L., and Liu, M. A. (1996) Protection against papillomavirus with a polynucleotide vaccine. J. Infect. Dis. 173, 314ā320.
Sundaram, P., Tigelaar, R. E., and Brandsma, J. L. (1997) Intracutaneous vaccination of rabbits with the cottontail rabbit papillomavirus (CRPV) L1 gene protects against virus challenge. (Erratum appears in Vaccine 1998;16: 655). Vaccine 15, 664ā671.
Koutsky, L. A., Ault, K. A., Wheeler, C. M., et al. (2002) A controlled trial of a human papillomavirus type 16 vaccine. N. Engl. J. Med. 347, 1645ā1651.
Ross, J. S., Fletcher, J. A., Bloom, K. J., et al. (2004) Targeted therapy in breast cancer: the HER-2/neu gene and protein. Mol. Cell Proteomics 3, 379ā398.
Lee, S. P., Brooks, J. M., Al-Jarrah, H., et al. (2004) CD8 T cell recognition of endogenously expressed epstein-barr virus nuclear antigen 1. J. Exper. Med. 199, 1409ā1420.
Roden, R. B., Ling, M., and Wu, T. C. (2004) Vaccination to prevent and treat cervical cancer. Hum. Pathol. 35, 971ā982.
Kang, K. K., Choi, S. M., Choi, J. H., et al. (2003) Safety evaluation of GX-12, a new HIV therapeutic vaccine: investigation of integration into the host genome and expression in the reproductive organs. Intervirology 46, 270ā276.
Osen, W., Peiler, T., Ohlschlager, P., et al. (2001) A DNA vaccine based on a shuffled E7 oncogene of the human papillomavirus type 16 (HPV 16) induces E7-specific cytotoxic T cells but lacks transforming activity. Vaccine 19, 4276ā4286.
Bisht, H., Roberts, A., Vogel, L., et al. (2004) Severe acute respiratory syndrome coronavirus spike protein expressed by attenuated vaccinia virus protectively immunizes mice. Proc. Natl. Acad. Sci. USA 101, 6641ā6646.
Fujii, T., Brandsma, J. L., Peng, X., et al. (2001) High and low levels of cottontail rabbit papillomavirus E2 protein generate opposite effects on gene expression. J. Biol. Chem. 276, 867ā874.
Brandsma, J. L., Shlyankevich, M., Zhang, L., et al. (2004) Vaccination of rabbits with an adenovirus vector expressing the papillomavirus E2 protein leads to clearance of papillomas and infection. J. Virol. 78, 116ā123.
McBride, A. A., Byrne, J. C., and Howley, P. M. (1989) E2 polypeptides encoded by bovine papillomavirus type 1 form dimers through the common carboxyl-terminal domain: transactivation is mediated by the conserved amino-terminal domain. Proc. Natl. Acad. Sci. USA 86, 510ā514.
Kozak, M. (1986) Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell 44, 283ā292.
Kim, C. H., Oh, Y., and Lee, T. H. (1997) Codon optimization for high-level expression of human erythropoietin (EPO) in mammalian cells. Gene 199, 293ā301.
OāHagan, D. T., Singh, M., Kazzaz, J., et al. (2002) Synergistic adjuvant activity of immunostimulatory DNA and oil/water emulsions for immunization with HIV p55 gag antigen. Vaccine 20, 3389ā3398.
Lundstrom, K. (2002) Alphavirus-based vaccines. Curr. Opin. Mol. Ther. 4, 28ā34.
Hung, C. F., Cheng, W. F., Chai, C. Y., et al. (2001) Improving vaccine potency through intercellular spreading and enhanced MHC class I presentation of antigen. J. Immunol. 166, 5733ā5740.
Kim, T. W., Hung, C. F., Kim, J. W., et al. (2004) Vaccination with a DNA vaccine encoding herpes simplex virus type 1 VP22 linked to antigen generates longterm antigen-specific CD8-positive memory T cells and protective immunity. Hum. Gene Ther. 15, 167ā177.
Chang, D. Z., Lomazow, W., Somberg, C. J., Stan, R., and Perales, M. A. (2004) Granulocyte-macrophage colony stimulating factor: an adjuvant for cancer vaccines. Hematology 9, 207ā215.
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Brandsma, J.L. (2006). DNA Vaccine Design. In: Saltzman, W.M., Shen, H., Brandsma, J.L. (eds) DNA Vaccines. Methods in Molecular Medicineā¢, vol 127. Humana Press. https://doi.org/10.1385/1-59745-168-1:3
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DOI: https://doi.org/10.1385/1-59745-168-1:3
Publisher Name: Humana Press
Print ISBN: 978-1-58829-484-5
Online ISBN: 978-1-59745-168-0
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