Skip to main content
SpringerLink
Log in
Book cover

DNA Vaccines pp 171–197Cite as

Prime-Boost Strategies in DNA Vaccines

  • Protocol

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 127))

Abstract

Induction of HIV-specific T-cell responses by vaccines may facilitate efficient control of HIV replication. Plasmid DNA vaccines and recombinant fowlpox virus (rFPV) vaccines are promising HIV-1 vaccine candidates, although delivering either vaccine alone may be insufficient to induce sufficient T-cell responses. A consecutive immunization strategy, known as “prime-boost,” involving priming with DNA and boosting with rFPV vaccines encoding multiple common HIV antigens, is used to induce broad and high-level T-cell immunity and ameliorate AIDS in macaques. This vaccine strategy is proceeding to clinical trials. This chapter describes the use of prime-boost vaccines to induce T-cell responses against HIV-1 and protective immunity against AIDS in macaques. Methods for the construction of the vaccines, the use of animal models, and the detection of immune responses are described.

This is a preview of subscription content, log in via an institution.

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Ulmer, J. B., Donnelly, J. J., Parker, S. E., et al. (1993) Heterologous protection against influenza by injection of DNA encoding a viral protein. Science 259, 1745–1749.

    Article  CAS  PubMed  Google Scholar 

  2. Liu, M. A. (2003) DNA vaccines: a review. J. Intern Med. 253, 402–410.

    Article  CAS  PubMed  Google Scholar 

  3. Dale, C. J., De Rose, R., Stratov, I., et al. (2004) Efficacy of DNA and fowlpoxvirus priming/boosting vaccines for simian-human immunodeficiency virus. J. Virol. 78, 13,819–13,828.

    Article  CAS  PubMed  Google Scholar 

  4. Dale, C. J., De Rose, R., Wilson, K. M., et al. (2004) Evaluation in macaques of HIV-1 DNA vaccines containing primate CpG motifs and fowlpoxvirus vaccines co-expressing IFNγ or IL-12. Vaccine 23, 188–197.

    Article  CAS  PubMed  Google Scholar 

  5. Kent, S. J., Zhao, A., Best, S. J., Chandler, J. D., Boyle, D. B., and Ramshaw, I. A. (1998) Enhanced T-cell immunogenicity and protective efficacy of a human immunodeficiency virus type 1 vaccine regimen consisting of consecutive priming with DNA and boosting with recombinant fowlpox virus. J. Virol. 72, 10,180–10,188.

    CAS  PubMed  Google Scholar 

  6. Leong, K. H., Ramsay, A. J., Morin, M. J., Robinson, H. L., Boyle, D. B., and Ramshaw, I. A. (1995) Generation of enhanced immune responses by consecutive immunisation with DNA and recombinant fowlpox viruses. Vaccines 95, 327–331.

    Google Scholar 

  7. Robinson, H. L., Montefiori, D. C., Johnson, R. P., et al. (1999) Neutralizing antibody-independent containment of immunodeficiency virus challenges by DNA priming and recombinant poxvirus booster immunizations. Nat. Med. 5, 526–534.

    Article  CAS  PubMed  Google Scholar 

  8. Amara, R. R., Villinger, F., Altman, J. D., et al. (2001) Control of a mucosal challenge and prevention of AIDS by a multiprotein DNA/MVA vaccine. Science 292, 69–74.

    Article  CAS  PubMed  Google Scholar 

  9. Allen, T. M., Vogel, T. U., Fuller, D. H., et al. (2000) Induction of AIDS virus-specific CTL activity in fresh, unstimulated peripheral blood lymphocytes from rhesus macaques vaccinated with a DNA prime/modified vaccinia virus Ankara boost regimen. J. Immunol. 164, 4968–4978.

    CAS  PubMed  Google Scholar 

  10. Wee, E. G., Patel, S., McMichael, A. J., and Hanke, T. (2002) A DNA/MVA-based candidate human immunodeficiency virus vaccine for Kenya induces multi-specific T cell responses in rhesus macaques. J. Gen. Virol. 83, 75–80.

    CAS  PubMed  Google Scholar 

  11. Shiver, J. W., Fu, T. M., Chen, L., et al. (2002) Replication-incompetent adenoviral vaccine vector elicits effective anti-immunodeficiency-virus immunity. Nature 415, 331–335.

    Article  CAS  PubMed  Google Scholar 

  12. McConkey, S. J., Reece, W. H., Moorthy, V. S., et al. (2003) Enhanced T-cell immunogenicity of plasmid DNA vaccines boosted by recombinant modified vaccinia virus Ankara in humans. Nat. Med. 9, 729–735.

    Article  CAS  PubMed  Google Scholar 

  13. Mwau, M., Cebere, I., Sutton, J., et al. (2004) A human immunodeficiency virus 1 (HIV-1) clade A vaccine in clinical trials: stimulation of HIV-specific T-cell responses by DNA and recombinant modified vaccinia virus Ankara (MVA) vaccines in humans. J. Gen. Virol. 85, 911–919.

    Article  CAS  PubMed  Google Scholar 

  14. Esparza, J. and Osmanov, S. (2003) HIV vaccines: a global perspective. Curr. Mol. Med. 3, 183–193.

    Article  CAS  PubMed  Google Scholar 

  15. Koup, R. A., Safrit, J. T., Cao, Y., et al. (1994) Temporal association of cellular immune responses with the initial control of viremia in primary human immunodeficiency virus type 1 syndrome. J. Virol. 68, 4650–4655.

    CAS  PubMed  Google Scholar 

  16. Schmitz, J. E., Kuroda, M. J., Santra, S., et al. (1999) Control of viremia in simian immunodeficiency virus infection by CD8+ lymphocytes. Science 283, 857–860.

    Article  CAS  PubMed  Google Scholar 

  17. Barouch, D. H., Santra, S., Schmitz, J. E., et al. (2000) Control of viremia and prevention of clinical AIDS in rhesus monkeys by cytokine-augmented DNA vaccination. Science 290, 486–492.

    Article  CAS  PubMed  Google Scholar 

  18. Goulder, P. J., Brander, C., Tang, Y., et al. (2001) Evolution and transmission of stable CTL escape mutations in HIV infection. Nature 412, 334–338.

    Article  CAS  PubMed  Google Scholar 

  19. O’Connor, D. H., Allen, T. M., Vogel, T. U., et al. (2002) Acute phase cytotoxic T lymphocyte escape is a hallmark of simian immunodeficiency virus infection. Nat. Med. 8, 493–499.

    Article  PubMed  Google Scholar 

  20. O’Connor, D. H., Mothe, B. R., Weinfurter, J. T., et al. (2003) Major histocompatibility complex class I alleles associated with slow simian immunodeficiency virus disease progression bind epitopes recognized by dominant acute-phase cytotoxic-T-lymphocyte responses. J. Virol. 77, 9029–9040.

    Article  PubMed  Google Scholar 

  21. Oxenius, A., Price, D. A., Trkola, A., et al. (2004) Loss of viral control in early HIV-1 infection is temporally associated with sequential escape from CD8+ T cell responses and decrease in HIV-1-specific CD4+ and CD8+ T cell frequencies. J. Infect. Dis. 190, 713–721.

    Article  CAS  PubMed  Google Scholar 

  22. Estcourt, M. J., Ramsay, A. J., Brooks, A., Thomson, S. A., Medveckzy, C. J., and Ramshaw, I. A. (2002) Prime-boost immunization generates a high frequency, high-avidity CD8(+) cytotoxic T lymphocyte population. Int. Immunol. 14, 31–37.

    Article  CAS  PubMed  Google Scholar 

  23. Kim, J. J., Yang, J. S., Montaner, L., Lee, D. J., Chalian, A. A., and Weiner, D. B. (2000) Coimmunization with IFN-gamma or IL-2, but not IL-13 or IL-4 cDNA can enhance Th1-type DNA vaccine-induced immune responses in vivo. J. Interferon Cytokine Res. 20, 311–319.

    Article  CAS  PubMed  Google Scholar 

  24. Boyer, J. D., Chattergoon, M., Muthumani, K., et al. (2002) Next generation DNA vaccines for HIV-1. J. Liposome Res. 12, 137–142.

    Article  CAS  PubMed  Google Scholar 

  25. Bertley, F. M., Kozlowski, P. A., Wang, S. W., et al. (2004) Control of simian/human immunodeficiency virus viremia and disease progression after IL-2-augmented DNA-modified vaccinia virus Ankara nasal vaccination in nonhuman primates. J. Immunol. 172, 3745–3757.

    CAS  PubMed  Google Scholar 

  26. Ranasinghe, C., Medveczky, C. J., Woltring, D. et al. (2006) Evaluation of fowlpox-vaccinia virus prime-boost vaccine strategies for high-level mucosal and systemic immunity against HIV-1 Vaccine in press.

    Google Scholar 

  27. Sambrook, J. E., Fritsch, F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.

    Google Scholar 

  28. Boyle, D. B., Anderson, M., Amos, R., Voysey, R., and Coupar, B. E. H. (2004) Construction of recombinant fowlpox viruses carrying multiple vaccine antigens and immunomodulatory molecules. BioTechniques. 37, 104–111.

    CAS  PubMed  Google Scholar 

  29. Rossio, J. L., Esser, M. T., Suryanarayana, K., et al. (1998) Inactivation of HIV-1 infectivity with preservation of conformational and functional integrity of virion surface proteins. J. Virol. 72, 7992–8001.

    CAS  PubMed  Google Scholar 

  30. Kent, S. J., Dale, C. J., Preiss, S., Mills, J., Campagna, D., and Purcell, D. F. J. (2001) Vaccination with attenuated simian immunodeficiency virus by DNA inoculation. J. Virol. 75, 11,930–11,934.

    Article  CAS  PubMed  Google Scholar 

  31. Thomson, S. A., Sherritt, M. A., Medveczky, J., et al. (1998) Delivery of multiple CD8 cytotoxic T cell epitopes by DNA vaccination. J. Immunol. 160, 1717–1723.

    CAS  PubMed  Google Scholar 

  32. Coupar, B. E., Teo, T., and Boyle, D. B. (1990) Restriction endonuclease mapping of the fowlpox virus genome. Virology 179, 159–167.

    Article  CAS  PubMed  Google Scholar 

  33. Silim, A., El Azhary, M. A., and Roy, R. S. (1982) A simple technique for preparation of chicken-embryo-skin cell cultures. Avian Dis. 26, 182–185.

    Article  CAS  PubMed  Google Scholar 

  34. Russell, N. D., Hudgens, M. G., Ha, R., Havenar-Daughton, C., and McElrath, M. J. (2003) Moving to human immunodeficiency virus type 1 vaccine efficacy trials: defining T cell responses as potential correlates of immunity. J. Infect. Dis. 187, 226–242.

    Article  CAS  PubMed  Google Scholar 

  35. Hudgens, M. G., Self, S. G., Chiu, Y. L., Russell, N. D., Horton, H., and McElrath, M. J. (2004) Statistical considerations for the design and analysis of the ELISpot assay in HIV-1 vaccine trials. J. Immunol. Methods 288, 19–34.

    Article  CAS  PubMed  Google Scholar 

  36. Maecker, H. T., Dunn, H. S., Suni, M. A., et al. (2001) Use of overlapping peptide mixtures as antigens for cytokine flow cytometry. J. Immunol Methods 255, 27–40.

    Article  CAS  PubMed  Google Scholar 

  37. Dale, C. J., Liu, X. S., De Rose, R., et al. (2002) Chimeric human papilloma virus-simian/human immunodeficiency virus virus-like-particle vaccines: immunogenicity and protective efficacy in macaques. Virology 301, 176–187.

    Article  CAS  PubMed  Google Scholar 

  38. Kent, S. J., Woodward, A., and Zhao, A. (1997) Human immunodeficiency virus type 1 (HIV-1)-specific T cell responses correlate with control of acute HIV-1 infection in macaques. J. Infect. Dis. 176, 1188–1197.

    Article  CAS  PubMed  Google Scholar 

  39. Nishimura, Y., Igarashi, T., Donau, O. K., et al. (2004) Highly pathogenic SHIVs and SIVs target different CD4+ T cell subsets in rhesus monkeys, explaining their divergent clinical courses. Proc. Natl. Acad. Sci. USA 101, 12,324–12,329.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia

    C. Jane Dale, Robert De Rose & Stephen J. Kent

  2. John Curtin School for Medical Research, Australian National University, Canberra, ACT, Australia

    Scott Thomson, Charani Ranasinghe, C. Jill Medveczky & Ian A. Ramshaw

  3. Primate Research Centre, Bogor Agricultural University, Bogor, Indonesia

    Joko Pamungkas

  4. CSIRO Livestock Industries, Geelong, Victoria, Australia

    David B. Boyle

Authors
  1. C. Jane Dale
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Scott Thomson
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Robert De Rose
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Charani Ranasinghe
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. C. Jill Medveczky
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Joko Pamungkas
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. David B. Boyle
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Ian A. Ramshaw
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Stephen J. Kent
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Biomedical Engineering, Yale University, New Haven, CT

    W. Mark Saltzman

  2. Department of Chemical Engineering, University of Washington, Seattle, WA

    Hong Shen

  3. Section of Comparative Medicine, Yale University, New Haven, CT

    Janet L. Brandsma

Rights and permissions

Reprints and permissions

Copyright information

© 2006 Humana Press Inc.

About this protocol

Cite this protocol

Dale, C.J. et al. (2006). Prime-Boost Strategies in DNA Vaccines. In: Saltzman, W.M., Shen, H., Brandsma, J.L. (eds) DNA Vaccines. Methods in Molecular Medicine™, vol 127. Humana Press. https://doi.org/10.1385/1-59745-168-1:171

Download citation

  • DOI: https://doi.org/10.1385/1-59745-168-1:171

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-484-5

  • Online ISBN: 978-1-59745-168-0

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation