Abstract
A capture enzyme-linked immunosorbent assay (ELISA) was optimized for identification of mouse adenovirus type 1 in infected brain homogenates. The ELISA method allows for a much faster quantitation of virus in infected organs than plaque assays. Methods for organ homogenization and the subsequent capture ELISA are described in this chapter.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Kajon, A. E., Brown, C. C., and Spindler, K. R. (1998) Distribution of mouse adenovirus type 1 in intraperitoneally and intranasally infected adult outbred mice. J. Virol. 72, 1219–1223.
Crowther, J. R. (ed.) (1995) ELISA: Theory and Practice. Humana Press, Totowa, NJ.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2007 Humana Press Inc.
About this protocol
Cite this protocol
Welton, A.R., Spindler, K.R. (2007). Capture ELISA Quantitation of Mouse Adenovirus Type 1 in Infected Organs. In: Wold, W.S.M., Tollefson, A.E. (eds) Adenovirus Methods and Protocols. Methods in Molecular Medicine, vol 130. Humana Press. https://doi.org/10.1385/1-59745-166-5:215
Download citation
DOI: https://doi.org/10.1385/1-59745-166-5:215
Publisher Name: Humana Press
Print ISBN: 978-1-58829-598-9
Online ISBN: 978-1-59745-166-6
eBook Packages: Springer Protocols