Abstract
Defective adenovirus deletion mutants can be grown by complementation in the presence of helper viruses that supply essential functions missing in the deletion mutant. In general, the deletion mutant then must be separated physically from the helper for use in subsequent experiments. This chapter includes suggestions for selection of helper viruses, protocols for the production of stocks by complementation, and procedures for physical separation of deletion mutants from their helpers.
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Ketner, G., Boyer, J. (2007). Isolation, Growth, and Purification of Defective Adenovirus Deletion Mutants. In: Wold, W.S.M., Tollefson, A.E. (eds) Adenovirus Methods and Protocols. Methods in Molecular Medicine, vol 130. Humana Press. https://doi.org/10.1385/1-59745-166-5:19
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DOI: https://doi.org/10.1385/1-59745-166-5:19
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Print ISBN: 978-1-58829-598-9
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