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Manipulation of Early Region 4

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Adenovirus Methods and Protocols

Part of the book series: Methods in Molecular Medicine ((MIMM,volume 130))

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Abstract

Adenovirus early region 4 (E4) regulates processes in infected cells that include viral late gene expression, nonhomologous end joining, responses to DNA damage, and apoptosis. E4 is essential for viral growth in most cell lines. In this chapter, the current knowledge of the functions of six E4 products is summarized briefly. Protocols are presented for manipulation of E4, incorporation of E4 mutations into the viral genome, and growth of E4 mutants on complementing cell lines. A compilation of the described E4-complementing cell lines is included.

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Author information

Authors and Affiliations

  1. Department of Genetic Medicine, Weill Medical College of Cornell University, New York, NY

    Julie Boyer PhD

  2. Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD

    Gary Ketner PhD

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  1. Julie Boyer PhD
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  2. Gary Ketner PhD
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Editor information

Editors and Affiliations

  1. Department of Molecular Microbiology and Immunology, Saint Louis University School of Medicine, St. Louis, Missouri

    William S. M. Wold  & Ann E. Tollefson  & 

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© 2007 Humana Press Inc.

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Boyer, J., Ketner, G. (2007). Manipulation of Early Region 4. In: Wold, W.S.M., Tollefson, A.E. (eds) Adenovirus Methods and Protocols. Methods in Molecular Medicine, vol 130. Humana Press. https://doi.org/10.1385/1-59745-166-5:1

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  • DOI: https://doi.org/10.1385/1-59745-166-5:1

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-598-9

  • Online ISBN: 978-1-59745-166-6

  • eBook Packages: Springer Protocols

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