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Production and Release Testing of Ovine Atadenovirus Vectors

  • Gerald W. Both
  • Fiona Cameron
  • Anne Collins
  • Linda J. Lockett
  • Jan Shaw
Protocol
Part of the Methods in Molecular Medicine book series (MIMM, volume 130)

Abstract

Gene-directed enzyme prodrug therapy (GDEPT) is an emerging approach for the treatment of cancers. A variety of viral vectors have been used to deliver genes that encode the relevant enzymes, and some have been tested in clinical trials. To ensure the potency and efficacy of such vectors and to obtain regulatory approval to administer them to humans, it is necessary to develop a suite of assays that provide quality assurance. New GDEPT vectors based on ovine atadenovirus and Escherichia coli purine nucleoside phosphorylase (PNP) have been developed for first time use in humans in a phase I trial for the treatment of prostate cancer. Here we describe methods for their production together with several quality-control assays. In particular, a functional cell killing assay was devised to measure the potency of PNP-GDEPT vectors, the principles of which could easily be adapted to other systems.

Key Words

Ovine atadenovirus vectors PNP-GDEPT prostate cancer 

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Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  • Gerald W. Both
    • 1
  • Fiona Cameron
    • 1
  • Anne Collins
    • 2
  • Linda J. Lockett
    • 1
  • Jan Shaw
    • 1
  1. 1.CSIRO Molecular and Health TechnologiesNorth RydeAustralia
  2. 2.Virient Party Ltd.ThebartonAustralia

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