Abstract
Mouse yeast artificial chromosomes (YACs) are useful platforms for manipulation of targeting vector design and construction, particularly in circumstances where polymerase chain reaction-mediated amplification of targeting arms proves fruitless or the cloned DNA is inherently unstable. This chapter describes a simple procedure where YACs can be modified to produce targeting vectors of various sizes.
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References
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© 2006 Humana Press Inc., Totowa NJ
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Murray, P.J. (2006). Targeting Vector Construction by Yeast Artificial Chromosome Modification. In: MacKenzie, A. (eds) YAC Protocols. Methods in Molecular Biology™, vol 349. Humana Press. https://doi.org/10.1385/1-59745-158-4:127
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DOI: https://doi.org/10.1385/1-59745-158-4:127
Publisher Name: Humana Press
Print ISBN: 978-1-58829-612-2
Online ISBN: 978-1-59745-158-1
eBook Packages: Springer Protocols