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YAC Protocols pp 187-197 | Cite as

Contribution of Yeast Artificial Chromosome-Based Physical Maps to the Final Assembly of the Trypanosoma cruzi Genome

  • Márcia R. M. Santos
  • Roberto R. M. Barros
  • Patrício E. Porcile
  • Mariano J. Levin
  • José Franco da Silveira
Part of the Methods in Molecular Biology™ book series (MIMB, volume 349)

Abstract

This chapter describes the methodology used both in performing the electrophoretic karyotype of the protozoan parasite Trypanosoma cruzi and mapping the genetic markers of the chromosomal bands, the construction of chromosome-specific YAC contigs, and their use to assign a chromosomal location to whole genome shotgun sequences.

Key Words

Trypanosoma cruzi protozoan parasite molecular karyotype chromosome-specific marker YAC whole genome shotgun (WGS) sequences integrated physical and WGS map 

References

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Copyright information

© Humana Press Inc., Totowa NJ 2006

Authors and Affiliations

  • Márcia R. M. Santos
    • 1
  • Roberto R. M. Barros
    • 1
  • Patrício E. Porcile
    • 1
  • Mariano J. Levin
    • 2
  • José Franco da Silveira
    • 1
  1. 1.Department of Microbiology, Immunology and ParasitologyEscola Paulista de Medicina, UNIFESPS. PauloBrazil
  2. 2.Institute of Genetic Engineering and Molecular BiologyBuenos AiresArgentina

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