Abstract
Because of technical hurdles, large-scale cell culture methods have not been widely exploited until recently for the study of Caenorhabditis elegans. Culturing differentiated cells from larvae and adult worms is probably not technically feasible because of difficulties in removing the animal’s cuticle and dissociating cells. In contrast, large numbers of developing embryo cells can be isolated relatively easily. When placed in culture, embryo cells undergo terminal differentiation within 24 h. Cultured embryo cells have been used recently to characterize ion channel function and regulation and to determine cell specific gene expression patterns. This chapter will provide a detailed description of the methods for isolating and culturing C. elegans embryo cells.
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References
Barr, M. M. (2003) Super models. Physiol. Genomics 13, 15–24.
Strange, K. (2003) From genes to integrative physiology: ion channel and transporter biology in Caenorhabditis elegans. Physiol. Rev. 83, 377–415.
Bloom, L. (1993) Genetic and molecular analysis of genes required for axon outgrowth in Caenorhabditis elegans, Massachusetts Institute of Technology, PhD thesis, Boston, MA, pp. 1–412.
Buechner, M., Hall, D.H., Bhatt, H., and Hedgecock, E. M. (1999) Cystic canal mutants in Caenorhabditis elegans are defective in the apical membrane domain of the renal (excretory) cell. Dev. Biol. 214, 227–241.
Christensen, M. and Strange, K. (2001) Developmental regulation of a novel outwardly rectifying mechanosensitive anion channel in Caenorhabditis elegans. J. Biol. Chem. 276, 45,024–45,030.
Christensen, M., Estevez, A. Y., Yin, X. M., et al. (2002) A primary culture system for functional analysis of C. elegans neurons and muscle cells. Neuron 33, 503–514.
Estevez, A. Y., Roberts, R. K., and Strange, K. (2003) Identification of store-independent and store-operated Ca2+ conductances in Caenorhabditis elegans intestinal epithelial cells. J. Gen. Physiol. 122, 207–223.
Yuan, A., Santi, C. M., Wei, A., et al. (2003) The sodium-activated potassium channel is encoded by a member of the Slo gene family. Neuron 37, 765–773.
Carvelli, L., McDonald, P. W., Blakely, R. D., and DeFelice, L. J. (2004) Dopamine transporters depolarize neurons by a channel mechanism. Proc. Natl. Acad. Sci. USA 101, 16,046–16,051.
Park, K. H., Hernandez, L., Cai, S. Q., Wang, Y., and Sesti, F. (2005) A family of K+ channel ancillary subunits regulate taste sensitivity in Caenorhabditis elegans. J. Biol. Chem. 280, 21,893–21,899.
Estevez, A. Y. and Strange, K. (2005) Calcium feedback mechanisms regulate oscillatory activity of a TRP-like Ca2+ conductance in C. elegans intestinal cells. J. Physiol. 567, 239–251.
Bianchi, L., Gerstbrein, B., Frokjaer-Jensen, C., et al. (2004) The neurotoxic MEC-4(d) DEG/ENaC sodium channel conducts calcium: implications for necrosis initiation. Nat. Neurosci. 7, 1337–1344.
Suzuki, H., Kerr, R., Bianchi, L., et al. (2003) In vivo imaging of C. elegans mechanosensory neurons demonstrates a specific role for the MEC-4 channel in the process of gentle touch sensation. Neuron 39, 1005–1017.
Teramoto, T., Lambie, E. J., and Iwasaki, K. (2005) Differential regulation of TRPM channels governs electrolyte homeostasis in the C. elegans intestine. Cell Metab. 1, 343–354.
Colosimo, M. E., Brown, A., Mukhopadhyay, S., et al. (2004) Identification of thermosensory and olfactory neuron-specific genes via expression profiling of single neuron types. Curr. Biol. 14, 2245–2251.
Cinar, H., Keles, S., and Jin, Y. (2005) Expression profiling of GABAergic motor neurons in Caenorhabditis elegans. Curr. Biol. 15, 340–346.
Fox, R. M., Von Stetina, S. E., Barlow, S. J., et al. (2005) A gene expression fingerprint of C. elegans embryonic motor neurons. BMC Genomics 6, 42.
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© 2006 Humana Press Inc.
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Strange, K., Morrison, R. (2006). In Vitro Culture of C. elegans Somatic Cells. In: Strange, K. (eds) C. elegans. Methods in Molecular Biology, vol 351. Humana Press. https://doi.org/10.1385/1-59745-151-7:265
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DOI: https://doi.org/10.1385/1-59745-151-7:265
Publisher Name: Humana Press
Print ISBN: 978-1-58829-597-2
Online ISBN: 978-1-59745-151-2
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