Abstract
Double-stranded RNA (dsRNA)-induced gene silencing in Caenorhabditis elegans involves the manufacture and delivery of defined sequences of dsRNA to the organism, followed by a careful monitoring for loss-of-function phenocopies in treated animals. In this chapter, we describe how to generate DNAs that can be used as templates for transcription of dsRNA.
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© 2006 Humana Press Inc.
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Timmons, L. (2006). Construction of Plasmids for RNA Interference and In Vitro Transcription of Double-Stranded RNA. In: Strange, K. (eds) C. elegans. Methods in Molecular Biology, vol 351. Humana Press. https://doi.org/10.1385/1-59745-151-7:109
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DOI: https://doi.org/10.1385/1-59745-151-7:109
Publisher Name: Humana Press
Print ISBN: 978-1-58829-597-2
Online ISBN: 978-1-59745-151-2
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