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C. elegans pp 119-125 | Cite as

Delivery Methods for RNA Interference in C. elegans

  • Lisa Timmons
Part of the Methods in Molecular Biology book series (MIMB, volume 351)

Abstract

This chapter describes four methods for delivery of double-stranded RNA to Caenorhabditis elegans (injection, feeding, soaking, and in vivo delivery), and suggests schemes that should facilitate detection of specific gene silencing.

Key Words

Double-stranded RNA dsRNA single-stranded RNA ssRNA RNAi RNA silencing feeding soaking injection HT115 HT115(DE3) transgene hairpin RNA RNaseIII transcription functional genomics 

References

  1. 1.
    Simmer, F., Tijsterman, M., Parrish, S., et al. (2002) Loss of the putative RNA-directed RNA polymerase RRF-3 makes C. elegans hypersensitive to RNAi. Curr. Biol. 12, 1317–1319.PubMedCrossRefGoogle Scholar
  2. 2.
    Kennedy, S., Wang, D., and Ruvkun, G. (2004) A conserved siRNA-degrading RNase negatively regulates RNA interference in C. elegans. Nature 427, 645–649.PubMedCrossRefGoogle Scholar
  3. 3.
    Kamath, R. S., Martinez-Campos, M., Zipperlen, P., Fraser, A. G., and Ahringer, J.(2001) Effectiveness of specific RNA-mediated interference through ingested double-stranded RNA in Caenorhabditis elegans. Genome Biol. 2, RESEARCH0002.Google Scholar
  4. 4.
    Hope, I. A. (1999) C. elegans: A Practical Approach, Oxford University Press, Oxford, UK.Google Scholar
  5. 5.
    Timmons, L., Court, D. L., and Fire, A. (2001) Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans. Gene 263, 103–112.PubMedCrossRefGoogle Scholar
  6. 6.
    Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E., and Mello, C. C. (1998) Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391, 806–811.PubMedCrossRefGoogle Scholar
  7. 7.
    Tabara, H., Grishok, A., and Mello, C. C. (1998) RNAi in C. elegans: soaking in the genome sequence. Science 282, 430–431.PubMedCrossRefGoogle Scholar
  8. 8.
    Maeda, I., Kohara, Y., Yamamoto, M., and Sugimoto, A. (2001) Large-scale analysis of gene function in Caenorhabditis elegans by high-throughput RNAi. Curr. Biol. 11, 171–176.PubMedCrossRefGoogle Scholar
  9. 9.
    Timmons, L. and Fire, A. (1998) Specific interference by ingested dsRNA. Nature 395, 854.PubMedCrossRefGoogle Scholar
  10. 10.
    Hull, D. and Timmons, L. (2004) Methods for delivery of double-stranded RNA into Caenorhabditis elegans. Methods Mol. Biol. 265, 23–584.PubMedGoogle Scholar
  11. 11.
    Epstein, H. F. and Shakes, D. C. (1995) Caenorhabditis elegans: Modern Biological Analysis of an Organism, Academic Press, New York, NY.Google Scholar

Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Lisa Timmons
    • 1
  1. 1.Department of Molecular BiosciencesUniversity of KansasLawrence

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