C. elegans pp 119-125 | Cite as

Delivery Methods for RNA Interference in C. elegans

  • Lisa Timmons
Part of the Methods in Molecular Biology book series (MIMB, volume 351)


This chapter describes four methods for delivery of double-stranded RNA to Caenorhabditis elegans (injection, feeding, soaking, and in vivo delivery), and suggests schemes that should facilitate detection of specific gene silencing.

Key Words

Double-stranded RNA dsRNA single-stranded RNA ssRNA RNAi RNA silencing feeding soaking injection HT115 HT115(DE3) transgene hairpin RNA RNaseIII transcription functional genomics 


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Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Lisa Timmons
    • 1
  1. 1.Department of Molecular BiosciencesUniversity of KansasLawrence

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