Abstract
A major feature in Dictyostelium development is the transition from the unicellular to the multicellular stage, a process brought about by chemotaxis and cell-cell adhesion. Growth-phase cells are weakly cohesive, whereas aggregation-competent cells adhere strongly to each other. In addition, aggregating cells display an ethylenediamine tetraacetic acid (EDTA)-resistant form of adhesion, which is developmentally regulated. Measuring cell-cell adhesion can thus be a simple and convenient method by which to assess the developmental progression of cells, to characterize mutants, and to discriminate between development and functional defects in cell adhesion molecules or membrane-cytoskeletal interactions. A quantitative cell adhesion assay is obviously crucial for identifying novel cell adhesion factors.
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© 2006 Humana Press Inc., Totowa, NJ
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Bozzaro, S. (2006). Assaying Cell-Cell Adhesion. In: Eichinger, L., Rivero, F. (eds) Dictyostelium discoideum Protocols. Methods in Molecular Biology™, vol 346. Humana Press. https://doi.org/10.1385/1-59745-144-4:449
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DOI: https://doi.org/10.1385/1-59745-144-4:449
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