The cDNA Sequencing Project

  • Hideko Urushihara
  • Takahiro Morio
  • Yoshimasa Tanaka
Part of the Methods in Molecular Biology™ book series (MIMB, volume 346)

Abstract

The Dictyostelium discoideum cDNA sequencing project started in 1995, preceding the genome sequencing project. Altogether, 14 cDNA libraries, including full-length ones, were constructed from five different stages of growth and asexual and sexual development, from which nearly 100,000 randomly chosen clones were sequenced to yield over 150,000 expressed sequence tags (ESTs). The data have been publicized online to facilitate clone distribution and collaboration using the whole clone set for microarray analyses. The EST reads were assembled to 6700 independent genes, which constitute about 55% of the total estimated Dictyostelium genes. Utilization of wet and dry resources have contributed to the understanding of the genetic system controlling the multicellular development in Dictyostelium.

Key Words

Dictyostelium discoideum development gene expression EST analysis DNA microarray in situ hybridization sequence database gene annotation promoter sequence 

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Copyright information

© Humana Press Inc., Totowa, NJ 2006

Authors and Affiliations

  • Hideko Urushihara
    • 1
  • Takahiro Morio
    • 1
  • Yoshimasa Tanaka
    • 1
  1. 1.Graduate School of Life and Environmental SciencesUniversity of TsukubaIbaraki-kenJapan

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