Abstract
Use of high copy number bacterial RNA offers several advantages in a diagnostics context compared with current deoxyribonucleic acid-based assays. The opportunity to only detect viable cells by targeting labile RNA transcripts may create an opportunity for “real-time” monitoring of pathogen load in response to a treatment regimen, while the natural amplification provided by the relative abundance of the RNA target compared with its corresponding gene opens a door to potential nonamplified direct detection technologies. In this chapter, a method is described to accurately quantify specific RNA transcripts and thus determine their potential utility as “high-copy” targets. The quantification method described also has application in gene-expression analysis.
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Glynn, B. (2006). Application of Two-Step Quantitative Reverse-Transcription PCR to Bacterial Diagnostics. In: O’Connor, L. (eds) Diagnostic Bacteriology Protocols. Methods in Molecular Biology™, vol 345. Humana Press. https://doi.org/10.1385/1-59745-143-6:97
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DOI: https://doi.org/10.1385/1-59745-143-6:97
Publisher Name: Humana Press
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