Abstract
The Naval Research Laboratory has developed an array-based biosensor system capable of detecting multiple pathogenic and toxic species in complex matrices. Sandwich fluoroimmunoassays are performed on the surface of a patterned microscope slide that acts as an optical waveguide. Fluorescence from immunocomplexes formed on the slide surface is excited using the evanescent field, an electromagnetic component of light, and the pattern of fluorescence is imaged using a charge-coupled device camera. Using the evanescent wave for excitation allows real-time imaging. Alternatively, a confocal scanner can also be used to detect and quantify fluorescent spots. A method for immobilizing capture antibodies, performing assays, and detecting bound targets is presented.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Knecht, B. G., Strasser, A., Dietrich, R., Martlbauer, E., Niessner, R., and Weller, M. G. (2004) Automated microarray system for the simultaneous detection of antibiotics in milk. Anal. Chem. 76, 646–654.
Rowe, C. A., Tender, L. M., Feldstein, M. J., et al. (1999) Array biosensor for simultaneous identification of bacterial, viral, and protein analytes. Anal. Chem. 71, 3846–3852.
Plowman, T. E., Durstchi, J. D., Wang, H. K., Christensen, D. A., Herron, J. N., and Reichert, W. M. (1999) Multiple-analyte fluoroimmunoassay using an integrated optical waveguide sensor. Anal. Chem. 71, 4344–4352
Sapsford, K. E., Shubin, Y. S., Delehanty, J. B., et al. (2004) Fluorescence-based array biosensor for detection of biohazards. J. Appl. Microbiol. 96, 47–58.
Rowe, C. A., Scruggs, S. B., Feldstein, M. J., Golden, J. P., and Ligler, F. S. (1999) An array immunosensor for the simultaneous detection of clinical analytes. Anal. Chem. 71, 433–439.
Rowe-Taitt, C. A., Hazzard, J. W., Hoffman, K. E., Cras, J. J., Golden, J. P., and Ligler, F. S. (2000) Simultaneous detection of six biohazardous agents using a planar waveguide array biosensor. Biosens. Bioelectron. 15, 579–589.
Delehanty, J. B. and Ligler, F. S. (2002) A microarray immunoassays for simultaneous detection of proteins and bacteria. Anal. Chem. 74, 5681–5687.
Sapsford, K. E., Ngundi, M. M., Moore, M. H., et al. (in press) Rapid detection of foodborne contaminants using an array biosensor. Sens. Actuators B.
Golden, J. P., Taitt, C. R., Shriver-Lake, L. C., Shubin, Y. S., and Ligler F. S. (2005) A portable automated multianalyte biosensor. Talanta. 65, 1078–1080.
Golden, J. P., Shriver-Lake, L. C., Taitt. C. R., Fertig, S., Sapsford, K. E., and Ligler, F. S. (2004) A portable array biosensor. Proc. SPIE 5586, 13–17.
Taitt, C. R., Golden, J. P., Shubin, Y. S., et al. (2004) A portable array biosensor for detecting multiple analytes in complex samples. Microbial. Ecol. 47, 175–185.
Golden, J. P., Ngundi, M. M., Shriver-Lake, L. C., Taitt, C. R., and Ligler, F. S. (2004) A portable array biosensor for food safety. Proc. SPIE 5587, 241–244.
Ngundi, M. M., Shriver-Lake, L. C., Moore, M. H., Lassman, M. E., Ligler, F. S., and Taitt, C. R. (2005) Array biosensor for detection of ochratoxin A in cereals and beverages. Anal. Chem. 77, 148–154.
Sapsford, K. E., Rasooly, A., Taitt, C. R., and Ligler, F. S. (2004) Detection of Campylobacter and Shigella species in food samples using an array biosensor. Anal. Chem. 76, 433–440.
Taitt, C. R., Shubin, Y. S., Angel, R., and Ligler, F. S. (2004) Detection of Salmonella enterica serovar Typhimurium by using a rapid, array-based immunosensor. Appl. Environ. Microbiol. 70, 152–158.
Shriver-Lake, L. C., Shubin, Y. S., and Ligler, F. S. (2003) Detection of staphylococcal enterotoxin B in spiked food samples. J. Food Protect. 66, 1851–1856.
Ligler, F. S., Taitt, C. R., Shriver-Lake L. C., et al. (2003) Array biosensor for detection of toxins. Anal. Bioanal. Chem. 377, 469–477.
Delehanty, J. B. and Ligler, F. S. (2003) A method for printing functional microarrays. BioTechniques 34, 380–385.
Sapsford, K. E., Charles, P. T., Patterson Jr, C. H., and Ligler, F. S. (2002) Demonstration of four immunoassay formats using the array biosensor. Anal. Chem. 74, 1061–1068.
Rowe-Taitt, C. A., Cras, J. J., Patterson, C. H., Golden, J. P, and Ligler, F. S. (2000) A ganglioside-based assay for cholera toxin using an array biosensor. Anal. Biochem. 281, 123–133.
Feldstein, M. J., Golden, J. P., Rowe, C. A., MacCraith, B. D., and Ligler, F. S. (1999) Array biosensor: optical and fluidic systems. J. Biomed. Microdevices 1, 139–153.
Golden J. P. and Ligler, F. S. (2002) A comparison of imaging methods for use in an array biosensor. Biosens. Bioelectron. 17, 719–725.
Sapsford, K. E., Liron, Z., Shubin, Y. S., and Ligler, F. S. (2001) Kinetics of antigen binding to arrays of antibodies in different sized spots. Anal. Chem. 73, 5518–5524.
Cras, J. J., Rowe-Taitt, C. A., Nivens, D. A., and Ligler, F. S. (1999) Comparison of the chemical cleaning methods of glass in preparation for silanization. Biosens. Bioelectron. 14, 683–688.
Dey, B. P. and Lattuada, C. P. (eds.) (1998) USDA/FSIS Microbiology Laboratory Guidebook, 3rd Ed., US Department of Agriculture, Washington, DC.
USDA (1995) Food safety and inspection service: Procedure for isolation of Salmonella from food. Laboratory Communication No. 75. US Department of Agriculture, Washington, DC.
US Food and Drug Administration (1998) Bacteriological Analytical Manual, 8th Ed. US Food and Drug Administration, Washington, DC.
Anderson, G. P. and Nerurkar, N. L. (2002) Improved fluoroimmunoassays using the dye Alexa Fluor 647 with the RAPTOR, a fiber optic biosensor. J. Immunol. Methods 271, 17–24.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2006 Humana Press Inc.
About this protocol
Cite this protocol
Ngundi, M.M., Taitt, C.R. (2006). An Array Biosensor for Detection of Bacterial and Toxic Contaminants of Foods. In: O’Connor, L. (eds) Diagnostic Bacteriology Protocols. Methods in Molecular Biology™, vol 345. Humana Press. https://doi.org/10.1385/1-59745-143-6:53
Download citation
DOI: https://doi.org/10.1385/1-59745-143-6:53
Publisher Name: Humana Press
Print ISBN: 978-1-58829-594-1
Online ISBN: 978-1-59745-143-7
eBook Packages: Springer Protocols