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Diagnostic Bacteriology Protocols pp 119–130Cite as

Amplified Fragment-Length Polymorphism and Protein Profiling for Identification of Campylobacter lari Subgroups

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 345))

Abstract

Amplified fragment-length polymorphism analysis (AFLP) has been shown to be a suitable method for subtyping of bacteria belonging to the genus Campylobacter. Campylobacter lari is a phenotypically and genotypically diverse species that comprises the classical nalidixic acid-resistant thermophilic campylobacters and the biochemical C. lari variants, urease-positive, nalidixic acid-susceptible, and urease producing nalidixic acid-susceptible strains. AFLP profiling and whole-cell protein profile analysis are suitable methods for studying the taxonomic and epidemiological relationships among strains of the C. lari variants. Numerical analysis of AFLP profiles and of partial protein profiles allows the discrimination of distinct C. lari genogroups. No correlation of these genogroups with different sources of the strains has been identified until now.

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Author information

Authors and Affiliations

  1. Department of Medical Microbiology, Academic Medical Center, Amsterdam, The Netherlands

    Birgitta Duim

  2. Animal Sciences Group, Lelystad, The Netherlands

    Jaap Wagenaar

Authors
  1. Birgitta Duim
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  2. Jaap Wagenaar
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Editor information

Editors and Affiliations

  1. National Diagnostic Centre and BioResearch Ireland, National University of Ireland, Galway, Ireland

    Louise O’Connor

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© 2006 Humana Press Inc.

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Duim, B., Wagenaar, J. (2006). Amplified Fragment-Length Polymorphism and Protein Profiling for Identification of Campylobacter lari Subgroups. In: O’Connor, L. (eds) Diagnostic Bacteriology Protocols. Methods in Molecular Biology™, vol 345. Humana Press. https://doi.org/10.1385/1-59745-143-6:119

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  • DOI: https://doi.org/10.1385/1-59745-143-6:119

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-594-1

  • Online ISBN: 978-1-59745-143-7

  • eBook Packages: Springer Protocols

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