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An Array Biosensor for Detection of Bacterial and Toxic Contaminants of Foods

  • Miriam M. Ngundi
  • Chris R. Taitt
Part of the Methods in Molecular Biology™ book series (MIMB, volume 345)

Abstract

The Naval Research Laboratory has developed an array-based biosensor system capable of detecting multiple pathogenic and toxic species in complex matrices. Sandwich fluoroimmunoassays are performed on the surface of a patterned microscope slide that acts as an optical waveguide. Fluorescence from immunocomplexes formed on the slide surface is excited using the evanescent field, an electromagnetic component of light, and the pattern of fluorescence is imaged using a charge-coupled device camera. Using the evanescent wave for excitation allows real-time imaging. Alternatively, a confocal scanner can also be used to detect and quantify fluorescent spots. A method for immobilizing capture antibodies, performing assays, and detecting bound targets is presented.

Key Words

Immunoassay immunosensor antibody-based detection food-borne contaminants rapid detection Salmonella Campylobacter staphylococcal enterotoxin B SEB 

References

  1. 1.
    Knecht, B. G., Strasser, A., Dietrich, R., Martlbauer, E., Niessner, R., and Weller, M. G. (2004) Automated microarray system for the simultaneous detection of antibiotics in milk. Anal. Chem. 76, 646–654.PubMedCrossRefGoogle Scholar
  2. 2.
    Rowe, C. A., Tender, L. M., Feldstein, M. J., et al. (1999) Array biosensor for simultaneous identification of bacterial, viral, and protein analytes. Anal. Chem. 71, 3846–3852.PubMedCrossRefGoogle Scholar
  3. 3.
    Plowman, T. E., Durstchi, J. D., Wang, H. K., Christensen, D. A., Herron, J. N., and Reichert, W. M. (1999) Multiple-analyte fluoroimmunoassay using an integrated optical waveguide sensor. Anal. Chem. 71, 4344–4352PubMedCrossRefGoogle Scholar
  4. 4.
    Sapsford, K. E., Shubin, Y. S., Delehanty, J. B., et al. (2004) Fluorescence-based array biosensor for detection of biohazards. J. Appl. Microbiol. 96, 47–58.PubMedCrossRefGoogle Scholar
  5. 5.
    Rowe, C. A., Scruggs, S. B., Feldstein, M. J., Golden, J. P., and Ligler, F. S. (1999) An array immunosensor for the simultaneous detection of clinical analytes. Anal. Chem. 71, 433–439.PubMedCrossRefGoogle Scholar
  6. 6.
    Rowe-Taitt, C. A., Hazzard, J. W., Hoffman, K. E., Cras, J. J., Golden, J. P., and Ligler, F. S. (2000) Simultaneous detection of six biohazardous agents using a planar waveguide array biosensor. Biosens. Bioelectron. 15, 579–589.PubMedCrossRefGoogle Scholar
  7. 7.
    Delehanty, J. B. and Ligler, F. S. (2002) A microarray immunoassays for simultaneous detection of proteins and bacteria. Anal. Chem. 74, 5681–5687.PubMedCrossRefGoogle Scholar
  8. 8.
    Sapsford, K. E., Ngundi, M. M., Moore, M. H., et al. (in press) Rapid detection of foodborne contaminants using an array biosensor. Sens. Actuators B.Google Scholar
  9. 9.
    Golden, J. P., Taitt, C. R., Shriver-Lake, L. C., Shubin, Y. S., and Ligler F. S. (2005) A portable automated multianalyte biosensor. Talanta. 65, 1078–1080.PubMedCrossRefGoogle Scholar
  10. 10.
    Golden, J. P., Shriver-Lake, L. C., Taitt. C. R., Fertig, S., Sapsford, K. E., and Ligler, F. S. (2004) A portable array biosensor. Proc. SPIE 5586, 13–17.CrossRefGoogle Scholar
  11. 11.
    Taitt, C. R., Golden, J. P., Shubin, Y. S., et al. (2004) A portable array biosensor for detecting multiple analytes in complex samples. Microbial. Ecol. 47, 175–185.CrossRefGoogle Scholar
  12. 12.
    Golden, J. P., Ngundi, M. M., Shriver-Lake, L. C., Taitt, C. R., and Ligler, F. S. (2004) A portable array biosensor for food safety. Proc. SPIE 5587, 241–244.CrossRefGoogle Scholar
  13. 13.
    Ngundi, M. M., Shriver-Lake, L. C., Moore, M. H., Lassman, M. E., Ligler, F. S., and Taitt, C. R. (2005) Array biosensor for detection of ochratoxin A in cereals and beverages. Anal. Chem. 77, 148–154.PubMedCrossRefGoogle Scholar
  14. 14.
    Sapsford, K. E., Rasooly, A., Taitt, C. R., and Ligler, F. S. (2004) Detection of Campylobacter and Shigella species in food samples using an array biosensor. Anal. Chem. 76, 433–440.PubMedCrossRefGoogle Scholar
  15. 15.
    Taitt, C. R., Shubin, Y. S., Angel, R., and Ligler, F. S. (2004) Detection of Salmonella enterica serovar Typhimurium by using a rapid, array-based immunosensor. Appl. Environ. Microbiol. 70, 152–158.PubMedCrossRefGoogle Scholar
  16. 16.
    Shriver-Lake, L. C., Shubin, Y. S., and Ligler, F. S. (2003) Detection of staphylococcal enterotoxin B in spiked food samples. J. Food Protect. 66, 1851–1856.Google Scholar
  17. 17.
    Ligler, F. S., Taitt, C. R., Shriver-Lake L. C., et al. (2003) Array biosensor for detection of toxins. Anal. Bioanal. Chem. 377, 469–477.PubMedCrossRefGoogle Scholar
  18. 18.
    Delehanty, J. B. and Ligler, F. S. (2003) A method for printing functional microarrays. BioTechniques 34, 380–385.PubMedGoogle Scholar
  19. 19.
    Sapsford, K. E., Charles, P. T., Patterson Jr, C. H., and Ligler, F. S. (2002) Demonstration of four immunoassay formats using the array biosensor. Anal. Chem. 74, 1061–1068.PubMedCrossRefGoogle Scholar
  20. 20.
    Rowe-Taitt, C. A., Cras, J. J., Patterson, C. H., Golden, J. P, and Ligler, F. S. (2000) A ganglioside-based assay for cholera toxin using an array biosensor. Anal. Biochem. 281, 123–133.PubMedCrossRefGoogle Scholar
  21. 21.
    Feldstein, M. J., Golden, J. P., Rowe, C. A., MacCraith, B. D., and Ligler, F. S. (1999) Array biosensor: optical and fluidic systems. J. Biomed. Microdevices 1, 139–153.CrossRefGoogle Scholar
  22. 22.
    Golden J. P. and Ligler, F. S. (2002) A comparison of imaging methods for use in an array biosensor. Biosens. Bioelectron. 17, 719–725.PubMedCrossRefGoogle Scholar
  23. 23.
    Sapsford, K. E., Liron, Z., Shubin, Y. S., and Ligler, F. S. (2001) Kinetics of antigen binding to arrays of antibodies in different sized spots. Anal. Chem. 73, 5518–5524.PubMedCrossRefGoogle Scholar
  24. 24.
    Cras, J. J., Rowe-Taitt, C. A., Nivens, D. A., and Ligler, F. S. (1999) Comparison of the chemical cleaning methods of glass in preparation for silanization. Biosens. Bioelectron. 14, 683–688.CrossRefGoogle Scholar
  25. 25.
    Dey, B. P. and Lattuada, C. P. (eds.) (1998) USDA/FSIS Microbiology Laboratory Guidebook, 3rd Ed., US Department of Agriculture, Washington, DC.Google Scholar
  26. 26.
    USDA (1995) Food safety and inspection service: Procedure for isolation of Salmonella from food. Laboratory Communication No. 75. US Department of Agriculture, Washington, DC.Google Scholar
  27. 27.
    US Food and Drug Administration (1998) Bacteriological Analytical Manual, 8th Ed. US Food and Drug Administration, Washington, DC.Google Scholar
  28. 28.
    Anderson, G. P. and Nerurkar, N. L. (2002) Improved fluoroimmunoassays using the dye Alexa Fluor 647 with the RAPTOR, a fiber optic biosensor. J. Immunol. Methods 271, 17–24.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Miriam M. Ngundi
    • 1
  • Chris R. Taitt
    • 1
  1. 1.Center for Biomolecular Science and EngineeringUS Naval Research LaboratoryWashington

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