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Amplified Fragment-Length Polymorphism and Protein Profiling for Identification of Campylobacter lari Subgroups

  • Birgitta Duim
  • Jaap Wagenaar
Part of the Methods in Molecular Biology™ book series (MIMB, volume 345)

Abstract

Amplified fragment-length polymorphism analysis (AFLP) has been shown to be a suitable method for subtyping of bacteria belonging to the genus Campylobacter. Campylobacter lari is a phenotypically and genotypically diverse species that comprises the classical nalidixic acid-resistant thermophilic campylobacters and the biochemical C. lari variants, urease-positive, nalidixic acid-susceptible, and urease producing nalidixic acid-susceptible strains. AFLP profiling and whole-cell protein profile analysis are suitable methods for studying the taxonomic and epidemiological relationships among strains of the C. lari variants. Numerical analysis of AFLP profiles and of partial protein profiles allows the discrimination of distinct C. lari genogroups. No correlation of these genogroups with different sources of the strains has been identified until now.

Key Words

Campylobacter lari AFLP typing genogroups protein profiling 

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Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Birgitta Duim
    • 1
  • Jaap Wagenaar
    • 2
  1. 1.Department of Medical MicrobiologyAcademic Medical CenterAmsterdamThe Netherlands
  2. 2.Animal Sciences GroupLelystadThe Netherlands

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