Abstract
Agrobacterium tumefaciens is best known for its ability to transform plants by delivering the T-DNA that is processed and transferred from the resident Ti plasmid to the recipient plant cells. Less well known is the capacity of this Gram-negative bacterium to transfer its T-DNA into fungi and actinomycetes. Procedures are described on the use of the promiscuous T-DNA transfer system of A. tumefaciens to transform members of the actinomycetes. Integration of the T-DNA derivatives into the Streptomyces chromosome provides opportunities of obtaining valuable mutants of this antibiotic producing soil organism.
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Kado, C.I., Kelly, B. (2006). Actinomycetes (Streptomyces lividans). In: Wang, K. (eds) Agrobacterium Protocols Volume 2. Methods in Molecular Biology, vol 344. Humana Press. https://doi.org/10.1385/1-59745-131-2:395
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DOI: https://doi.org/10.1385/1-59745-131-2:395
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