Actinomycetes (Streptomyces lividans)

  • Clarence I. Kado
  • Brian Kelly
Part of the Methods in Molecular Biology book series (MIMB, volume 344)


Agrobacterium tumefaciens is best known for its ability to transform plants by delivering the T-DNA that is processed and transferred from the resident Ti plasmid to the recipient plant cells. Less well known is the capacity of this Gram-negative bacterium to transfer its T-DNA into fungi and actinomycetes. Procedures are described on the use of the promiscuous T-DNA transfer system of A. tumefaciens to transform members of the actinomycetes. Integration of the T-DNA derivatives into the Streptomyces chromosome provides opportunities of obtaining valuable mutants of this antibiotic producing soil organism.

Key Words

Streptomyces pUCD2614 high copy vir helper plasmid pUCD2335 plant transformation vector pUCD5801 Streptomyces transformation vector 


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Copyright information

© Humana Press Inc., Totowa, NJ 2006

Authors and Affiliations

  • Clarence I. Kado
    • 1
  • Brian Kelly
    • 1
  1. 1.Davis Crown Gall GroupUniversity of CaliforniaDavis

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