Actinomycetes (Streptomyces lividans)
Agrobacterium tumefaciens is best known for its ability to transform plants by delivering the T-DNA that is processed and transferred from the resident Ti plasmid to the recipient plant cells. Less well known is the capacity of this Gram-negative bacterium to transfer its T-DNA into fungi and actinomycetes. Procedures are described on the use of the promiscuous T-DNA transfer system of A. tumefaciens to transform members of the actinomycetes. Integration of the T-DNA derivatives into the Streptomyces chromosome provides opportunities of obtaining valuable mutants of this antibiotic producing soil organism.
Key WordsStreptomyces pUCD2614 high copy vir helper plasmid pUCD2335 plant transformation vector pUCD5801 Streptomyces transformation vector
- 7.Kelly, B. and Kado, C.I. (1997) Promiscuous gene transfer of Agrobacterium tumefaciens extends to the actinomycete Streptomyces lividans. Amer. Soc. Microbiol. 97th Annu. Mtg. Abstr. p. 417.Google Scholar
- 14.Dworkin, M.M., Falkow, S., Rosenberg, E., Schleifer K.-H., and Stacebrandt, E. (eds.). (2005) The Prokaryotes, Third Edition, vol. 3, Archaea and Bacteria: Firmicutes, Actinomycetes. Springer, Heidelberg, Germany, p. 998.Google Scholar
- 20.Kieser, T., Bibb, M.J., Buttner, M.J., Chater, K.F., and Hopwood, D.A. (eds.) (2000) Practical Streptomyces Genetics. The John Innes Foundation, Norwich, UK.Google Scholar
- 21.Hopwood, D.A., Bibb, M.J., Chater, K.F., et al. (eds.) (1985) Genetic Manipulation of Streptomyces: A Laboratory Manual, John Innes Foundation, Norwich, UK.Google Scholar