Coffee (Coffea sp.)

  • Thierry Leroy
  • Edgardo Alpizar
  • Magali Dufour
  • Hervé Etienne
Part of the Methods in Molecular Biology book series (MIMB, volume 344)

Abstract

Coffee (Coffea sp.) is a perennial plant widely cultivated in many tropical countries. It is a cash crop for millions of small farmers in these areas. As compared with other tree species, coffee has long breeding cycles that make conventional breeding programs time consuming. For that matter, genetic transformation can be an effective technique to introduce a desired trait in an already “elite” variety, or to study a gene function and expression. In this chapter, we describe two Agrobacterium-mediated transformation techniques; the first with A. tumefaciens to introduce an insect resistance gene and the second with A. rhizogenes to study candidate gene expression for nematode resistance in transformed roots.

Key Words

Agrobacterium tumefaciens Agrobacterium rhizogenes insect resistance nematode resistance Bacillus thuringensis Meloidogyne sp. functional genomics 

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Copyright information

© Humana Press Inc., Totowa, NJ 2006

Authors and Affiliations

  • Thierry Leroy
    • 1
  • Edgardo Alpizar
    • 1
  • Magali Dufour
    • 1
  • Hervé Etienne
    • 1
  1. 1.Centre de Coopération Internationale en Recherche Agronomique pour le Développment, Départment des Cultures Pérennes (CIRAD-CP), TA 80/03Montpellier Cedex 5France

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